The content of ursolic acid and oleanolic acid was determined in different plant parts of two Glechoma species, G. hederacea and G. hirsuta. To achieve optimal extraction conditions of ursolic acid and oleanolic acid from plant material, several methods including maceration, heat reflux, Soxhlet, and ultrasonic extraction, as well as various solvents (methanol, dichloromethane, ethyl acetate), were investigated and compared.For the simultaneous quantification of pentacyclic triterpenes in extracts from Glechoma sp., an UPLC-MS/MS was developed and validated. The method exhibited good linearity, precision, and recovery, and it also was simple, specific, and fast. We developed the method for future application in the quality control of plant materials and botanical extracts containing ursolic acid and oleanolic acid. With regard to the triterpene constituents, both G. hederacea and G. hirsuta can be used equally, and the aboveground parts of both species, but the leaves especially, are abundant sources of ursolic acid (7.1 - 7.5 mg/g dry weight [DW]). Dichloromethane as an extractant provided the best extraction efficiency as well as selectivity to obtain Glechoma extracts rich in triterpenes as compared to methanol and ethyl acetate, regardless of the particular extraction technique. Dry dichloromethane extracts from aerial parts of Glechoma sp. obtained by the heat reflux method resulted in products with a high content of UA (17 - 25% w/w) are considered to be convenient and rich sources of this compound.

中文翻译：

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通过 UPLC/MS/MS 同时定量 Glechoma hederacea 和 Glechoma hirsuta 中的熊果酸和齐墩果酸

测定了两种 Glechoma 物种 G. hederacea 和 G. hirsuta 的不同植物部位的熊果酸和齐墩果酸的含量。为了从植物材料中获得熊果酸和齐墩果酸的最佳提取条件，研究和比较了几种方法，包括浸渍、热回流、索氏提取和超声波提取，以及各种溶剂（甲醇、二氯甲烷、乙酸乙酯）。为同时定量 Glechoma sp. 提取物中的五环三萜，开发并验证了 UPLC-MS/MS。该方法具有良好的线性、精密度和回收率，而且方法简单、特异性强、快速。我们开发了未来在含有熊果酸和齐墩果酸的植物材料和植物提取物的质量控制中应用的方法。关于三萜成分，G. hederacea 和 G. hirsuta 都可以同等使用，并且这两个物种的地上部分，尤其是叶子，是熊果酸的丰富来源（7.1 - 7.5 mg/g 干重 [DW] ]）。与甲醇和乙酸乙酯相比，二氯甲烷作为萃取剂可提供最佳的萃取效率和选择性，以获得富含三萜类的山茶花萃取物，无论采用何种特定的萃取技术。来自 Glechoma sp. 地上部分的干燥二氯甲烷提取物。通过热回流法获得的产物中 UA 含量高 (17 - 25% w/w) 被认为是该化合物的方便和丰富的来源。是熊果酸的丰富来源（7.1 - 7.5 毫克/克干重 [DW]）。与甲醇和乙酸乙酯相比，二氯甲烷作为萃取剂可提供最佳的萃取效率和选择性，以获得富含三萜类的山茶花萃取物，无论采用何种特定的萃取技术。来自 Glechoma sp. 地上部分的干燥二氯甲烷提取物。通过热回流法获得的产物中 UA 含量高 (17 - 25% w/w) 被认为是该化合物的方便和丰富的来源。是熊果酸的丰富来源（7.1 - 7.5 毫克/克干重 [DW]）。与甲醇和乙酸乙酯相比，二氯甲烷作为萃取剂可提供最佳的萃取效率和选择性，以获得富含三萜类的山茶花萃取物，无论采用何种特定的萃取技术。来自 Glechoma sp. 地上部分的干燥二氯甲烷提取物。通过热回流法获得的产物中 UA 含量高 (17 - 25% w/w) 被认为是该化合物的方便和丰富的来源。来自 Glechoma sp. 地上部分的干燥二氯甲烷提取物。通过热回流法获得的产物中 UA 含量高 (17 - 25% w/w) 被认为是该化合物的方便和丰富的来源。从 Glechoma sp. 地上部分提取的干燥二氯甲烷提取物。通过热回流法获得的产物中 UA 含量高 (17 - 25% w/w) 被认为是该化合物的方便和丰富的来源。