Depletion of CTCF disrupts PSG gene expression in the human trophoblast cell line Swan 71,FEBS Open Bio

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Depletion of CTCF disrupts PSG gene expression in the human trophoblast cell line Swan 71
FEBS Open Bio ( IF 2.8 ) Pub Date : 2021-01-16 , DOI: 10.1002/2211-5463.13087
Da Som Jeong _{1,

2} , Myoung Hee Kim _{1,

2} , Ji-Yeon Lee ₁

Affiliation

Pregnancy‐specific glycoproteins (PSGs) are fetal proteins secreted by the placenta during pregnancy. The PSG level in maternal serum is an indicator of risk for pregnancy complications. However, little is known about the molecular mechanisms underlying PSG gene expression. Recently, the importance of epigenetic regulation of placental genes has been emphasized in the study of developmental defects and placental disease. In this study, the role of the CCCTC‐binding factor (CTCF) in regulation of PSG expression was investigated to better understand the epigenetic regulatory mechanisms of the PSG genes. Inhibition of CTCF expression disturbed transcription of several PSG genes: PSG1, PSG2, PSG4, PSG5, PSG8, and PSG9 were upregulated and PSG6 and PSG11 were downregulated. These transcriptional changes were correlated with decreased CTCF binding and changes in histone modification at the PSG promoters. Our data demonstrate that CTCF is a potential mediator in the regulation of PSG gene expression.

中文翻译：

CTCF 的消耗破坏了人滋养层细胞系 Swan 71 中 PSG 基因的表达

妊娠特异性糖蛋白 (PSG) 是妊娠期间胎盘分泌的胎儿蛋白质。母体血清中的 PSG 水平是妊娠并发症风险的指标。然而，人们对 PSG 基因表达的分子机制知之甚少。最近，胎盘基因表观遗传调控的重要性在发育缺陷和胎盘疾病的研究中得到了强调。在这项研究中，研究了 CCCTC 结合因子（CTCF）在 PSG 表达调控中的作用，以更好地了解 PSG 基因的表观遗传调控机制。CTCF 表达的抑制干扰了几个 PSG 基因的转录：PSG1、PSG2、PSG4、PSG5、PSG8和PSG9上调，PSG6和PSG11下调。这些转录变化与 CTCF 结合减少和 PSG 启动子处组蛋白修饰的变化相关。我们的数据表明 CTCF 是调节 PSG 基因表达的潜在介质。

更新日期：2021-03-04

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