Dynamic imaging of zebrafish heart with multi‐planar light sheet microscopy,Journal of Biophotonics

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Dynamic imaging of zebrafish heart with multi‐planar light sheet microscopy
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2021-01-16 , DOI: 10.1002/jbio.202000466
Zengxin Huang ₁ , Panchun Gu ₁ , Dengfeng Kuang ₁ , Ping Mi ₂ , Xizeng Feng ₂

Affiliation

Light sheet fluorescence microscopy has become a research hotspot in biomedicine because of low phototoxicity, high speed, and high resolution. However, the conventional methods to acquire three‐dimensional spatial information are mainly based on scanning, which inevitably increases photodamage and is not real‐time. Here, we propose a method to generate controllable multi‐planar illumination with a dielectric isosceles triangular array and a design of multi‐planar light sheet fluorescence microscopy system. We carry out experiments of three‐dimensional illumination beam measurement, volumetric imaging of fluorescent microspheres, and dynamic in vivo imaging of zebrafish heart to evaluate the performance of this system. In addition, we apply this system to study the effects of bisphenol fluorene on the heart shape and heart‐beating rate of zebrafish. Our experiment results indicate that the multi‐planar light sheet microscopy system provides a novel and feasible method for three‐dimensional selected plane imaging and low‐phototoxicity in vivo imaging.

中文翻译：

斑马鱼心脏的动态成像与多平面光片显微镜

由于光毒性低，速度快和分辨率高，光片荧光显微镜已成为生物医学的研究热点。但是，获取三维空间信息的常规方法主要是基于扫描，这不可避免地会增加光损伤，而且不是实时的。在这里，我们提出了一种利用介电等腰三角形阵列产生可控的多平面照明的方法，以及一种多平面光片荧光显微镜系统的设计。我们进行了三维照明束测量，荧光微球体成像和斑马鱼心脏动态体内成像实验，以评估该系统的性能。此外，我们应用该系统研究双酚芴对斑马鱼心形和心跳率的影响。我们的实验结果表明，多平面光片显微镜系统为三维选择平面成像和体内低光毒性成像提供了一种新颖可行的方法。

更新日期：2021-01-16

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