• The signaling pathway mediated by the receptor‐like kinase ERECTA (ER) plays important roles in plant immune responses, but the underlying mechanism is unclear.
• Genetic interactions between ER signaling and the chromatin remodeling complex SWR1 in the control of plant immune responses were studied. Electrophoretic mobility shift assay and yeast one‐hybrid analysis were applied to identify ER‐WRKY33 downstream components. Chromatin immunoprecipitation analyses were further investigated.
• In this study, we show that the chromatin remodeling complex SWR1 enhances resistance to the white mold fungus Sclerotinia sclerotiorum in Arabidopsis thaliana via a process mediated by ER signaling. We identify a series of WRKY33 target YODA DOWNSTREAM (YDD) genes and demonstrate that SWR1 and ER signaling are required to enrich H2A.Z histone variant and H3K4me3 histone modification at YDDs and the binding of WRKY33 to YDD promoters upon S. sclerotiorum infection. We also reveal that the binding of WRKY33 to YDD promoters in turn promotes the enrichment of H2A.Z and H3K4me3 at YDD genes, thereby forming a positive regulatory loop to activate YDDs expression.
• Our study reveals how H2A.Z, H3K4me3 and ER signaling mutually regulate YDDs gene expression upon pathogen infection, highlighting the critical role of chromatin structure in ER‐signaling‐mediated plant immune responses.

中文翻译：

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ERECTA信号传导通过染色质介导的WRKY33与靶基因的结合而调节植物的免疫反应

• 受体样激酶ERECTA（ER）介导的信号通路在植物免疫反应中起重要作用，但其潜在机制尚不清楚。
• 研究了ER信号转导和染色质重塑复合体SWR1在植物免疫反应控制中的遗传相互作用。电泳迁移率变动分析和酵母一杂交分析用于鉴定ER-WRKY33下游成分。进一步研究了染色质的免疫沉淀分析。
• 在这项研究中，我们表明，染色质结构重塑复杂SWR1提高对白色霉菌抗菌核病在拟南芥中通过由ER信号介导的过程。我们鉴定了一系列WRKY33目标YODA下流（YDD）基因，并证明SWR1和ER信号转导需要富集H2A.Z组蛋白变体和H3K4me3组蛋白修饰在YDD s和WRKY33到SDD硬化菌后YDD启动子的结合。我们还揭示了WRKY33与YDD启动子的结合反过来促进了YDD处H2A.Z和H3K4me3的富集基因，从而形成一个正调节环来激活YDD的表达。
• 我们的研究揭示了H2A.Z，H3K4me3和ER信号转导如何在病原体感染后相互调节YDD s基因表达，突显了染色质结构在ER信号介导的植物免疫反应中的关键作用。