Owing to its superior bioactivity, biocompatibility, and biodegradability, bioactive glass (BG) has been attracting significant attention within biomedical science fields. However, BG powders with variable particle sizes are required targeting different applications, such as dermal fillers. Thus, in this study, granulated BG powders were prepared via the spray drying and granulation method. Size-controlled BG micron sphere with diameters of up to tens of microns can be mass-produced and their phase information, particle morphology, and specific surface area were characterized via X-ray diffraction, scanning electron microscopy, and nitrogen adsorption/desorption isotherm, respectively. In addition, the in vitro bioactivity was evaluated following Kokubo's protocol, and the formation of a hydroxyapatite (HA) layer after immersion into the simulated body fluid was confirmed via energy-dispersive and Fourier transform infrared spectroscopy. The cytotoxicity was examined using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Finally, the resulting morphologies and corresponding properties are shown, and the related mechanisms are discussed.

中文翻译：

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喷雾干燥颗粒状生物活性玻璃微球的制备与表征

由于其优越的生物活性、生物相容性和生物降解性，生物活性玻璃（BG）在生物医学科学领域引起了极大的关注。但是，需要针对不同应用（例如真皮填充剂）使用具有可变粒径的 BG 粉末。因此，在本研究中，通过喷雾干燥和造粒方法制备了颗粒状 BG 粉末。可以批量生产直径达数十微米的尺寸可控的 BG 微米球，并通过 X 射线衍射、扫描电子显微镜和氮吸附/解吸等温线表征它们的相信息、颗粒形态和比表面积，分别。此外，按照 Kokubo 的方案评估了体外生物活性，并且通过能量色散和傅里叶变换红外光谱证实了在浸入模拟体液后形成了羟基磷灰石 (HA) 层。使用溴化 3-(4, 5-二甲基噻唑-2-基)-2, 5-二苯基四唑鎓测定法检查细胞毒性。最后，显示了所得的形态和相应的特性，并讨论了相关的机制。