An extraordinary degree of condensation is required to fit the eukaryotic genome inside the nucleus. This compaction is attained by first coiling the DNA around structures called nucleosomes. Mammalian genomes are further folded into sophisticated three‐dimensional (3D) configurations, enabling the genetic code to dictate a diverse range of cell fates. Recent advances in molecular and computational technologies have enabled the query of higher‐order chromatin architecture at an unprecedented resolution and scale. In T lymphocytes, similar to other developmental programs, the hierarchical genome organization is shaped by a highly coordinated division of labor among different classes of sequence‐specific transcription factors. In this review, we will summarize the general principles of 1D and 3D genome organization, introduce the common experimental and computational techniques to measure the multilayer chromatin organization, and discuss the pervasive role of transcription factors on chromatin organization in T lymphocytes.

中文翻译：

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通过转录因子之间的分工重塑 T 淋巴细胞中的染色质景观

核内的真核基因组需要高度的凝聚。这种压实是通过首先将 DNA 缠绕在称为核小体的结构周围来实现的。哺乳动物基因组进一步折叠成复杂的三维 (3D) 配置，使遗传密码能够决定各种细胞命运。分子和计算技术的最新进展使得以前所未有的分辨率和规模查询高阶染色质结构成为可能。在 T 淋巴细胞中，与其他发育程序类似，分层基因组组织是由不同类别的序列特异性转录因子之间高度协调的分工形成的。在这篇综述中，我们将总结 1D 和 3D 基因组组织的一般原则，