Leptospirosis is an endemic disease caused by Leptospira spp., a bacterium that affects animals and humans. In recent years, the number of reports of leptospirosis in wild animals has increased, which highlights the need to study the infectious agents in these animals. In this study, a duplex PCR for the detection of leptospiral DNA was performed on 50 kidney samples from bats, and a MAT (Microscopic Agglutination Test) for serological detection of anti-leptospiral antibodies was applied to 47 serum samples from bats from different regions of Buenos Aires Province, Argentina.

DNA was extracted using Chelex-100 and duplex PCR was performed by targeting the detection of genes secY and flaB, of pathogenic Leptospira spp. Of the 50 kidney samples, 3 were positive for Eumops sp. and Tadarida brasiliensis by duplex PCR. Of the 47 serum samples, 12 were positive for different serovars: Leptospira interrogans serovars Icterohaemorrhagiae, Cynopteri and Bataviae, and Leptospira borgpetersenii serovar Ballum. This is the first report of the detection of pathogenic leptospires by serology in bats belonging to the T. brasiliensis and Eptesicus furinalis species in Argentina. In addition, this is the first report of the detection of pathogenic leptospiral DNA by PCR in T. brasiliensis species. The detection of Leptospira spp. in these wild animals shows that they may play an important role as wildlife reservoirs of leptospires.

钩端螺旋体病是由钩端螺旋体属（一种影响动物和人类的细菌）引起的地方病。近年来，野生动物钩端螺旋体病的报告数量有所增加，这凸显了研究这些动物的传染源的必要性。本研究对 50 份蝙蝠肾脏样本进行了双链 PCR 检测钩端螺旋体 DNA 的检测，并对 47 份来自不同地区的蝙蝠的血清样本进行了 MAT（显微凝集试验）抗钩端螺旋体抗体的血清学检测。阿根廷布宜诺斯艾利斯省。

使用 Chelex-100 提取 DNA，并通过靶向检测致病钩端螺旋体属的基因secY和flaB进行双链 PCR 。在 50 个肾脏样本中，3 个对Eumops sp.呈阳性。和Tadarida brasiliensis通过双链 PCR。在 47 份血清样本中，有 12 份不同血清型呈阳性：问号钩端螺旋体黄疸出血型、Cynopteri 和 Bataviae 血清型，以及波氏钩端螺旋体血清型Ballum。这是通过血清学在属于T. brasiliensis和Eptesicus furinalis 的蝙蝠中检测致病性钩端螺旋体的第一份报告阿根廷的物种。此外，这是首次通过 PCR 技术检测巴西毛钩端螺旋体病原性钩端螺旋体 DNA 的报道。钩端螺旋体属的检测。在这些野生动物中表明，它们可能作为野生动物的钩端虫储存库发挥重要作用。