Signal dependent microbial communication in Pseudomonas aeruginosa PAO1 is a typical phenomenon mediated by acyl homo-serine lactone molecules that helps in developing biofilm and enhance antibiotic resistance. Microbial sources provide insight to the hidden treasure of secondary metabolites, and these structurally diversified chemical motifs can be used as antimicrobial and anti-infective agents. In the present study, endophytic fungus, Colletotrichum gloeosporioides isolated from Carica papaya leaves explored for anti-infective potential against P. aeruginosa PAO1. The crude extract of C. gloeosporioides displayed bacteriostatic effect on P. aeruginosa PAO1 growth at 750 μg/ml concentration. A significant decline was observed in the production of quorum sensing regulated virulence factors, i.e. 56.32%, 62.54%, and 66.67% of pyocyanin, chitinase, and elastase enzyme, respectively. A drastic reduction in pathogenic determinant behaviour after treatment with crude extract of C. gloeosporioides i.e. EPS, rhamnolipid, and HCN production was noted. Light microscopy and CLSM analysis revealed that fungal extract treatment has reduced bacterial ability to form dense biofilm architecture. In silico analysis demonstrated the binding efficiency of bioactive compound, 4-(2,3-dimethoxybenzylidene)-3-methyl-1-(4-nitrophenyl)-2-pyrazolin-5-one, which is equipotent to the natural ligand and displayed a docking score of −5.436 kcal/mol with QS transcriptional regulator (LasR). Whereas the compound Acetamide, n-[tetrahydro-3-(phenylmethyl) thieno [3,4-d]thiazol-2 (3 h)-ylidene]-, s,s-dioxide exhibits a docking score of −4.088 kcal/mol (LasR) and −1.868 kcal/mol (RhlR) with cognate receptor proteins. Henceforth, the research report suggests C. gloeosporioides derived metabolites could be considered as a potential inhibitors of QS regulated virulence factors and biofilm production in P. aeruginosa PAO1.

铜绿假单胞菌PAO1中的信号依赖性微生物通讯是由酰基高丝氨酸内酯分子介导的典型现象，有助于形成生物膜并增强抗生素抗性。微生物来源提供了对次生代谢产物隐藏宝藏的洞察力，这些结构多样的化学基序可以用作抗菌剂和抗感染剂。在本研究中，探索从番木瓜叶中分离出的内生真菌Colletotrichum gloeosporioides对铜绿假单胞菌PAO1具有抗感染潜力。C. gloeosporioides的粗提物对铜绿假单胞菌显示出抑菌作用PAO1在750μg/ ml浓度下生长。在群体感应调节的毒力因子的产生中观察到了显着下降，即黄绿色素，几丁质酶和弹性蛋白酶的分别为56.32％，62.54％和66.67％。注意到在用球孢梭菌粗提物（即EPS，鼠李糖脂和HCN产生）处理后，致病决定因素的行为急剧减少。光学显微镜和CLSM分析表明，真菌提取物处理降低了细菌形成致密生物膜结构的能力。电脑分析表明生物活性化合物4-（2,3-二甲氧基亚苄基）-3-甲基-1-（4-硝基苯基）-2-吡唑啉-5-酮的结合效率与天然配体等价并显示出对接QS转录调节剂（LasR）的评分为−5.436 kcal / mol。而化合物乙酰胺，正-[四氢-3-（苯基甲基）噻吩并[3,4-d]噻唑-2（3h）-亚烷基]-，s，s-二氧化物的对接得分为-4.088 kcal / mol （LasR）和−1.868 kcal / mol（RhlR），具有同源受体蛋白。今后，该研究报告表明，球孢梭菌衍生的代谢产物可被认为是铜绿假单胞菌PAO1中QS调节的致病因子和生物膜产生的潜在抑制剂。