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Generating a fucose permease deletion mutant in Bifidobacterium longum subspecies infantis ATCC 15697
Anaerobe ( IF 2.5 ) Pub Date : 2021-01-16 , DOI: 10.1016/j.anaerobe.2021.102320
Melanie A Higgins 1 , Katherine S Ryan 1
Affiliation  

Bifidobacterium longum subsp. infantis ATCC 15697 has emerged as a model for infant gut-associated bifidobacterial strains. Here we present a genetic system for B. longum subsp. infantis ATCC 15697 using its own DNA restriction-modification systems and create a fucose permease deletion mutant lacking the ability to use free fucose as a carbon source.



中文翻译:

在长双歧杆菌婴儿亚种 ATCC 15697 中生成岩藻糖通透酶缺失突变体

长双歧杆菌亚种。infantis ATCC 15697 已成为婴儿肠道相关双歧杆菌菌株的模型。在这里,我们提出了B. longum subsp的遗传系统。infantis ATCC 15697 使用其自己的 DNA 限制性修饰系统,并创建一个缺乏使用游离岩藻糖作为碳源的能力的岩藻糖通透酶缺失突变体。

更新日期:2021-01-18
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