Malaria is the world's deadliest parasitic disease. Great progress has been made in the fight against malaria over the past two decades, but this has recently begun to plateau, in part due to the global development of antimalarial drug resistance. The ability to track drug resistance is necessary to achieve progress in treatment, disease surveillance and epidemiology, which has prompted the development of advanced diagnostic methods. These new methods provide unprecedented access to information that can help to guide public health policies. Development of new technologies increases the potential for high throughput and reduced costs of diagnostic tests; improving the accessibility of tools to investigate the forces driving disease dynamics and, ultimately, clinical outcomes for malaria patients and public health. This literature review provides a summary of the methods currently available for the detection of antimalarial drug resistance from the examination of patients’ blood samples. While no single method is perfect for every application, many of the newly developed methods give promise for more reliable and efficient characterisation of Plasmodium resistance in a range of settings. By exploiting the strengths of the tools available, we can develop a deeper understanding of the evolutionary and spatiotemporal dynamics of this disease. This will translate into more effective disease control, better-informed policy, and more timely and successful treatment for malaria patients.

疟疾是世界上最致命的寄生虫病。在过去的二十年中，在抗击疟疾方面取得了长足的进步，但由于全球抗疟药物耐药性的发展，这种情况最近已开始趋于平稳。追踪耐药性的能力对于实现治疗，疾病监测和流行病学的进步是必不可少的，这促使了高级诊断方法的发展。这些新方法为信息提供了前所未有的途径，可以帮助指导公共卫生政策。新技术的开发增加了高吞吐量的潜力，并降低了诊断测试的成本；改善工具的可及性，以调查驱动疾病动态的因素，并最终调查疟疾患者和公共卫生的临床结果。这篇文献综述总结了目前可通过检查患者血液样本来检测抗疟药耐药性的方法。尽管没有一种方法适合每种应用，但许多新开发的方法都有望实现更可靠，更高效的表征。耐疟原虫的设置范围。通过利用可用工具的优势，我们可以对这种疾病的进化和时空动态有更深入的了解。这将转化为更有效的疾病控制，更明智的政策以及对疟疾患者的更及时和成功的治疗。