The accumulation of nitrogen and phosphorous has detrimental effects on aquatic animals and negatively influences the economic benefits of aquaculture; thus, the control of water quality is of significance for the development of the aquaculture industry. In this study, a coculture system of Chlorella vulgaris + Rhodosporidium sphaerocarpum exhibited superior nitrogen removal performances compared with those of either the Isochrysis galbana + R. sphaerocarpum or the Tetraselmis helgolandica + R. sphaerocarpum coculture systems. The alginate-immobilized C. vulgaris and suspended R. sphaerocarpum coculture system was advantageous in terms of nutrient removal and the inoculum ratio (IR, dry weight of C. vulgaris to dry weight of R. sphaerocarpum) of 5:1 was determined to yield optimal removal performances of nutrients and the chemical oxygen demand (COD), with which 86.20% of the NH₄⁺ content was removed, and 100.00% removal efficiency of NO₂⁻, NO₃⁻, PO₄³⁻, and COD was detected after 120 h of incubation. The growth of C. vulgaris and R. sphaerocarpum was enhanced and a biomass productivity of 0.22 g L⁻¹ day⁻¹ was obtained. The sustainability of the optimized microalgae-yeast coculture system was confirmed by steady and consistent removal performances in semicontinuous experiments, removing over 80% of NH₄⁺, 100% of NO₃⁻, and over 90% of NO₂⁻, PO₄³⁻, and COD. These results indicated that the optimized microalgae-yeast coculture system developed in our studies might provide a potential method for the biological treatment of aquaculture wastewater.

氮和磷的积累对水生动物有不利影响，并对水产养殖的经济效益产生不利影响；因此，控制水质对水产养殖业的发展具有重要意义。在这项研究中，共培养系统小球藻+红冬sphaerocarpum与任一相比，显示出优异的脱氮性能等鞭金藻+ R. sphaerocarpum或扁藻helgolandica + R. sphaerocarpum共培养系统。藻酸盐固定化的寻常梭状芽胞杆菌和悬浮的球形芽孢杆菌共培养系统在去除养分方面是有利的，并且接种物比率（IR，菜豆的干重与球形橡胶的干重）确定为5：1，以产生最佳的养分去除性能和化学需氧量（COD） ），与该NH的86.20％₄ ⁺内容已被移除，和NO的100.00％去除率₂ ^-，NO ₃ ^-，PO ₄ ^3-，和COD被温育120小时后进行检测。寻常小球藻和球形果的生长得到增强，生物量生产力为0.22 g L ^-1 day ^-1获得了。优化的微藻酵母共培养系统的可持续性通过在半连续实验稳定和一致的去除性能，去除NH超过80％的证实₄ ⁺，NO的100％的₃ ^- ，和超过90％的NO的₂ ^-，PO ₄ ^{3 -}和COD。这些结果表明，我们研究中开发的优化的微藻-酵母共培养系统可能为水产养殖废水的生物处理提供潜在的方法。