The concentrations of ATP, IL-6, and IL-10 were measured in extracts of plucked hair follicles from healthy volunteers (normal values) and patients with androgenetic alopecia and then, ATP, IL-6, and IL-10 content was calculated for each follicle. The resulting values were directly proportional to hair follicle length, except for IL-6. The concentration of extracted ATP correlated with lactate dehydrogenase activity indicating cell damage. In patients with androgenetic alopecia, IL-10 content exceeded the normal values in follicles with a length <1 mm and ATP content surpassed the normal in follicles >2 mm long. The content of IL-6 and IL-10 measured by ELISA was comparable with results of mRNA expression assayed by RT-PCR, which attested to moderate level of gene expression. The content of ATP and IL- 10, but not IL-6 depended on the length of plucked hair follicle and on pathogenetic factors affecting hair growth.

中文翻译：

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拔毛毛囊中细胞因子和ATP的分析

从健康志愿者（正常值）和雄激素性脱发患者的毛囊提取物中测量 ATP、IL-6 和 IL-10 的浓度，然后计算 ATP、IL-6 和 IL-10 的含量每个毛囊。结果值与毛囊长度成正比，IL-6 除外。提取的 ATP 浓度与指示细胞损伤的乳酸脱氢酶活性相关。在雄激素性脱发患者中，IL-10含量在长度<1mm的毛囊中超过正常值，ATP含量在>2mm长的毛囊中超过正常值。ELISA 测定的 IL-6 和 IL-10 含量与 RT-PCR 测定的 mRNA 表达结果相当，证明基因表达水平中等。ATP和IL-10的含量，