Opportunistic microorganisms in the gut biocenosis were studied in adolescents with normal body weight and obesity (patients consulted at the Clinical Department of Research Center of Family Health and Human Reproduction Problems). The biological material was studied by standard bacteriological methods, representatives of Enterobacteriaceae family were also characterized using metagenomic sequencing of V3-V4 variable regions of 16S gene rRNA. Gut microbiota of obese adolescents was unbalanced and was characterized by low levels of bifido- and lactoflora representatives, a spectrum of E. coli associations, and high prevalence of opportunistic microorganisms and their associations. Representatives of Enterobacteriaceae family were most often found in the gut microbiota of obese adolescents.

中文翻译：

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肥胖青少年机会性微生物群和肠道生物群落中 16S rRNA 基因 V3-V4 可变区的元测序

在正常体重和肥胖的青少年（在家庭健康和人类生殖问题研究中心临床部咨询的患者）中研究了肠道生物群落中的机会微生物。通过标准细菌学方法研究生物材料，还使用 ​​16S 基因 rRNA 的 V3-V4 可变区的宏基因组测序对肠杆菌科的代表进行了表征。肥胖青少年的肠道微生物群是不平衡的，其特点是双歧杆菌和乳酸菌代表水平低、大肠杆菌关联范围广，以及机会性微生物及其关联的高流行率。肠杆菌科的代表最常见于肥胖青少年的肠道微生物群中。