The alphabaculoviruses are lethal pathogens of lepidopteran caterpillars including a polyphagous and globally recognized pest, Helicoverpa armigera (Hubner) infesting economically important agriculture crops worldwide. The biological and molecular characterizations of indigenous nucleopolyhedrovirus of the genus Alphabaculovirus isolated from H. armigera in chickpea fields are described. The virulence of virus isolate was tested in 3rd instar H. armigera larvae, and LC50 (median lethal concentration) was estimated to be 2.69 × 104 OBs ml−1. The ST50 (median survival time) was 4 days post-inoculation, when the 3rd instar H. armigera larvae were inoculated by OB (occlusion body) concentration equivalent to LC90. An average incubation period of the virus isolate in 3rd instar ranged between 4 and 6 days post-inoculation. The OBs of a virus isolate appeared irregular in shape and variable in size with diameter ranging from 0.57 to 1.46 μm on the longest edge and average of 1.071 ± 0.068 μm (mean ± SE). On the basis of phylogenetic analysis of polh, pif-1, and lef-8 genes, the isolate was found to be a member of the genus Alphabaculovirus. The isolate showed a genetic affinity with species of group II Alphabaculoviruses and appeared to be a group II NPV. On the basis of molecular phylogeny and associated host insect, this indigenous isolate was designated as HearNPV-IIPR05 isolate, which could be a potential candidate for the biological control of H. armigera infesting legumes and other commercial crops.

中文翻译：

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杆状病毒的特征：感染棉铃虫幼虫的HearNPV-IIPR05分离株

杆状病毒是鳞翅目毛虫的致病性病原体，包括多食性和全球公认的害虫Helicoverpa armigera（Hubner），在世界范围内具有重要的经济作物。描述了从鹰嘴豆田中的棉铃虫中分离出的杆状病毒属原生核多角体病毒的生物学和分子特征。在三龄棉铃虫幼虫中测试了病毒分离物的毒力，LC50（致死中值）估计为2.69×104 OBs ml-1。ST3（中位生存时间）是接种后4天，当时用等于LC90的OB（闭塞体）浓度接种3龄棉铃虫幼虫。病毒分离株在第三龄期的平均潜伏期为接种后4至6天。病毒分离株的OB形状不规则，大小可变，最长边缘的直径范围为0.57至1.46μm，平均值为1.071±0.068μm（平均值±SE）。根据polh，pif-1和lef-8基因的系统发育分析，发现分离株是Alphabaculovirus属的成员。该分离物显示出与II型杆状病毒种的遗传亲和力，并且似乎是II型NPV。根据分子系统发育学和相关寄主昆虫，这种本地分离株被称为HearNPV-IIPR05分离株，它可能是对棉铃虫侵染性豆类和其他商业作物进行生物防治的潜在候选者。根据polh，pif-1和lef-8基因的系统发育分析，发现分离株是Alphabaculovirus属的成员。该分离物显示出与II型杆状病毒种的遗传亲和力，并且似乎是II型NPV。根据分子系统发育学和相关寄主昆虫，这种本地分离株被称为HearNPV-IIPR05分离株，它可能是对棉铃虫侵染性豆类和其他商业作物进行生物防治的潜在候选者。根据polh，pif-1和lef-8基因的系统发育分析，发现分离株是Alphabaculovirus属的成员。该分离物显示出与II型杆状病毒种的遗传亲和力，并且似乎是II型NPV。根据分子系统发育学和相关寄主昆虫，这种本地分离株被称为HearNPV-IIPR05分离株，它可能是对棉铃虫侵染性豆类和其他商业作物进行生物防治的潜在候选者。