A rapid and simple approach for the characterization and quantification of gold nanoparticles in cell culture medium by single particle-ICP-MS,Journal of Analytical Atomic Spectrometry

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A rapid and simple approach for the characterization and quantification of gold nanoparticles in cell culture medium by single particle-ICP-MS
Journal of Analytical Atomic Spectrometry ( IF 3.1 ) Pub Date : 2020-12-22 , DOI: 10.1039/d0ja00441c
Sergio Fernández-Trujillo _{1,

2,

3,

4,

5} , María Jiménez-Moreno _{1,

2,

3,

4,

5} , Ángel Ríos _{1,

3,

5,

6,

7} , Rosa del Carmen Rodríguez Martín-Doimeadios _{1,

2,

3,

4,

5}

Affiliation

A rapid and simple methodology based on single particle-ICP-MS (SP-ICP-MS) has been developed for the study of gold nanoparticles (AuNPs) of different sizes in a cell culture medium commonly used in toxicological studies (Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and antibiotics as it is used in cell culture). Key instrumental parameters for the signal acquisition in single particle mode (e.g. dwell time and acquisition time) have been optimized. This new approach accomplishes the characterization and quantification of AuNPs in only one minute of analysis at concentrations in the ng L⁻¹ range with a minimum sample preparation because only dilution was required. Precise determinations of particle size and concentration (%RSD around 1%) were reported with 22.4 nm and 2.1 × 10⁵ particles per L as limit of detection for particle size (LOD_size) and for particle-number concentration (LOD_NP), respectively. AuNPs were incubated with DMEM at 37 °C at different times up to 96 h to study the possible transformations undergone in this medium. The incubation time had an effect on the concentrations (number- and mass-based), even though different trends for AuNP concentrations over time were observed depending on the AuNP size. However, no differences were observed regarding the particle size.

中文翻译：

通过单颗粒ICP-MS快速，简单地表征和定量细胞培养基中金纳米颗粒的方法

已经开发了一种基于单颗粒ICP-MS（SP-ICP-MS）的快速简便的方法，用于研究毒理学研究常用的细胞培养基（Dulbecco的改良Eagle培养基）中不同大小的金纳米颗粒（AuNP）。（DMEM）补充了10％的胎牛血清（FBS）和抗生素（用于细胞培养）。用于单粒子模式下信号采集的关键仪器参数（例如停留时间和采集时间）已经过优化。在ng L ^-1的浓度下，这种新方法仅需一分钟即可完成AuNPs的表征和定量由于仅需要稀释，因此样品制备量最少。据报道，精确测定粒径和浓度（％RSD约为1％）时，每L的检测极限分别为22.4 nm和2.1×10 ^5个粒子（LOD_尺寸）和颗粒数浓度（LOD _NP）。。将AuNP与DMEM在37°C下于不同时间孵育长达96小时，以研究在该培养基中可能发生的转化。孵育时间会影响浓度（基于数量和质量），即使根据AuNP大小观察到的AuNP浓度随时间变化的趋势也不同。但是，没有观察到粒径差异。

更新日期：2021-01-15

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