A 1-mo-old reticulated giraffe had progressive anorexia and died at the Ordos Zoo. Autopsy revealed necrotic stomatitis with severe bilateral necroulcerative lesions at the base of the tongue and of the cheeks near the commissures of the mouth. There was also severe bilateral confluent bronchopneumonia with a pronounced bronchial pattern and multifocal fibrinous pleuritis. Histologically, there was serofibrinous-suppurative bronchopneumonia with necrosuppurative bronchiolitis and necrotic arteritis. Filamentous bacteria with morphology consistent with Fusobacterium necrophorum were observed at the advancing edge of the necrotic tissue in the tongue and cheeks, as well as in the affected alveolar spaces and bronchioles. Aggregates of slender, gram-negative, rod-like or filamentous bacteria were identified in the lung impression smear. PCR results of 16S rDNA of the tongue and lung lesions had 100% homology to the F. necrophorum subsp. funduliforme B35 sequence (EF447425.1). The gross, histologic, Gram stain, and PCR product sequencing features in our case were consistent with oral and pulmonary necrobacillosis in ruminants, a rare disease of giraffes.

一只1个月大的网纹长颈鹿患有进行性厌食症，死于鄂尔多斯动物园。尸检显示坏死性口炎，在舌根和靠近嘴连合处的脸颊处有严重的双侧坏死性溃疡性病变。还存在严重的双侧融合性支气管肺炎，具有明显的支气管模式和多灶性纤维蛋白性胸膜炎。组织学上，有血清纤维蛋白性化脓性支气管肺炎伴坏死性化脓性细支气管炎和坏死性动脉炎。形态与坏死梭杆菌一致的丝状细菌在舌头和脸颊的坏死组织的前进边缘以及受影响的肺泡空间和细支气管中观察到。在肺印模涂片中鉴定出细长、革兰氏阴性、棒状或丝状细菌的聚集体。舌和肺损伤的 16S rDNA 的 PCR 结果与F. necrophorum subsp. 具有 100% 的同源性。眼底形B35 序列 (EF447425.1) 。在我们的病例中，大体、组织学、革兰氏染色和 PCR 产物测序特征与反刍动物口腔和肺坏死杆菌病一致，这是一种罕见的长颈鹿疾病。