Objective Intestinal flora and metabolites are associated with multiple systemic diseases. Current approaches for acquiring information regarding microbiota/metabolites have limitations. We aimed to develop a precise magnetically controlled sampling capsule endoscope (MSCE) for the convenient, non-invasive and accurate acquisition of digestive bioinformation for disease diagnosis and evaluation. Design The MSCE and surgery were both used for sampling both jejunal and ileal GI content in the control and antibiotic-induced diarrhoea groups. The GI content was then used for microbiome profiling and metabolomics profiling. Results Compared with surgery, our data showed that the MSCE precisely acquired data regarding the intestinal flora and metabolites, which was effectively differentiated in different intestinal regions and disease models. Using MSCE, we detected a dramatic decrease in the abundance of Bacteroidetes, Patescibacteria and Actinobacteria and hippuric acid levels, as well as an increase in the abundance of Escherichia–Shigella and the 2-pyrrolidinone levels were detected in the antibiotic-induced diarrhoea model by MSCE. MSCE-mediated sampling revealed specific gut microbiota/metabolites including Enterococcus , Lachnospiraceae , acetyl-L-carnitine and succinic acid, which are related to metabolic diseases, cancers and nervous system disorders. Additionally, the MSCE exhibited good sealing characteristics with no contamination after sampling. Conclusions We present a newly developed MSCE that can non-invasively and accurately acquire intestinal bioinformation via direct visualization under magnetic control, which may further aid in disease prevention, diagnosis, prognosis and treatment. Data are available upon reasonable request.

中文翻译：

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磁控采样胶囊内窥镜无创肠道生物信息采集的新方案

目的肠道菌群和代谢产物与多种全身性疾病有关。当前获取有关微生物群/代谢物信息的方法存在局限性。我们的目标是开发一种精确的磁控采样胶囊内窥镜（MSCE），用于方便、无创、准确地获取消化系统生物信息，用于疾病诊断和评估。设计 MSCE 和手术均用于对对照组和抗生素引起的腹泻组的空肠和回肠 GI 含量进行采样。然后将 GI 含量用于微生物组分析和代谢组学分析。结果与手术相比，我们的数据显示MSCE精确获取了关于肠道菌群和代谢物的数据，这些数据在不同的肠道区域和疾病模型中有效区分。使用MSCE，我们检测到拟杆菌属、Patescibacteria 和 Actinobacteria 的丰度以及马尿酸水平的显着下降，以及 Escherichia-Shigella 的丰度和 MSCE 在抗生素诱导的腹泻模型中检测到的 2-吡咯烷酮水平的增加。MSCE 介导的采样揭示了特定的肠道微生物群/代谢物，包括肠球菌、毛螺菌科、乙酰左旋肉碱和琥珀酸，它们与代谢疾病、癌症和神经系统疾病有关。此外，MSCE 表现出良好的密封特性，采样后无污染。结 预后和治疗。可根据合理要求提供数据。