Summary In vitro production of embryos has gained prominence as a tool for use in wildlife conservation programmes in situ and ex situ. However, the development of this technique depends on steps that include ovarian stimulation, collection and oocyte maturation. The purpose of this study was to assess the feasibility of an ovarian stimulation protocol for follicular aspiration, the efficiency of videolaparoscopy for follicular aspiration and test a medium for in vitro oocyte maturation for the species Mazama gouazoubira. Five females were submitted to repeated ovarian stimulation (hormone protocol using controlled internal drug release), and estradiol benzoate on D0 and eight injections of follicle-stimulating hormone, once every 12 h, from D4 onwards at 30-day intervals. Fourteen surgical procedures were performed in superstimulated females, resulting in the collection of 94 oocytes and an average of 17.1 ± 9.1 follicles observed, 13.5 ± 6.6 follicles aspirated and 7.2 ± 3.7 oocytes collected per surgery. After collection, the oocytes were submitted to in vitro maturation for 24 h and stained with Hoechst 33342 dye to evaluate their nuclear status; 64.5% of the oocytes reached MII and 16.1% were spontaneously activated by parthenogenesis. The nuclear status of oocytes that did not undergo in vitro maturation was evaluated; 80.9% were found to be immature.

中文翻译：

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卵巢刺激后获得的 Mazama gouazoubira（棕色小鹿）卵母细胞的收集和体外成熟

概括体外胚胎生产已成为野生动物保护计划中使用的一种工具原位和异地. 然而，这项技术的发展取决于包括卵巢刺激、收集和卵母细胞成熟在内的步骤。本研究的目的是评估用于卵泡抽吸的卵巢刺激方案的可行性、视频腹腔镜检查用于卵泡抽吸的效率并测试用于体外该物种的卵母细胞成熟马扎马古阿祖比拉. 五名女性接受重复的卵巢刺激（使用受控内部药物释放的激素方案），在第 0 天接受苯甲酸雌二醇和 8 次注射促卵泡激素，每 12 小时一次，从第 4 天开始，每隔 30 天一次。对过度刺激的女性进行了 14 次手术，结果收集了 94 个卵母细胞，平均观察到 17.1 ± 9.1 个卵泡，每次手术吸出 13.5 ± 6.6 个卵泡和 7.2 ± 3.7 个卵母细胞。收集后，将卵母细胞提交至体外成熟 24 小时并用 Hoechst 33342 染料染色以评估其核状态；64.5% 的卵母细胞达到 MII，16.1% 的卵母细胞通过孤雌生殖自发激活。未经历的卵母细胞的核状态体外评估成熟度；80.9%被发现是不成熟的。