Summary In this study, we tried to maintain the vitality of Chinese sturgeon (Acipenser sinensis) ova before fertilization with several treatments in vitro. The ovulated eggs were allocated to groups with different incubation medium (coelomic fluid and artificial media), temperature (4°C and 16°C) and storage duration (2 h and 6 h). The maximum fertilization and hatching rate were observed for the control group in which the ova were fertilized immediately after spawning, with the values of 82.45% and 84.73%, respectively. Compared with the control group, the fertilization and hatching rate of all the treatment groups stored at 4°C or in coelomic fluid decreased significantly (P < 0.05). The fertilization rate of the treatment group stored in artificial medium at 16°C did not change obviously in the first 2 h (P > 0.05), but declined dramatically (P < 0.05) after 6 h. In comparison with the control group, no significant (P > 0.05) reduction was shown in hatching rate of the treatment group stored in artificial medium at 16°C for 6 h. The results showed that the ova of Chinese sturgeon can be stored for at least 6 h at 16°C in artificial medium without weakening; this provides a practical application method for the routine hatchery practice of Chinese sturgeon, as well as certain relevant research.

中文翻译：

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中华鲟（Acipenser sinensis）卵子的体外短期储存

小结 在本研究中，我们试图保持中华鲟的生命力（中华鲟) 受精前的卵子经过多次处理体外. 将排卵的卵分配到具有不同孵化介质（体腔液和人工介质）、温度（4°C 和 16°C）和储存时间（2 小时和 6 小时）的组中。产卵后立即受精的对照组受精率和孵化率最高，分别为82.45%和84.73%。与对照组相比，4℃或体腔液贮藏各处理组的受精率和孵化率均显着降低（磷< 0.05)。处理组16℃人工培养基贮藏后2 h受精率无明显变化（磷> 0.05)，但急剧下降 (磷< 0.05) 6 小时后。与对照组相比，无显着性（磷> 0.05) 显示在 16°C 人工培养基中储存 6 小时的处理组的孵化率降低。结果表明，中华鲟的卵在16℃人工培养基中至少可以保存6小时而不变弱；这为中华鲟常规孵化实践以及一定的相关研究提供了切实可行的应用方法。