Hepatocellular carcinoma (HCC) is one of the most common types of cancer that is associated with poor quality of life in patients and a global health burden. The mechanisms involved in the development and progression of HCC remain poorly understood.

Hepatocellular carcinoma human samples and cell lines were subjected to qRT-PCR for expression assessment. CCK-8 assay, Transwell migration and invasion assay, were applied for cell function detection. Animal experiment was used to measure the function of SNHG17 on cell growth in vivo. Western blot was conducted to evaluate the level of EMT in cells. RIP, RNA pull-down and luciferase reporter assays were performed to assess the correlation between SNHG17, miR-3180-3p and RFX1.

Our study demonstrated that SNHG17 was upregulated in HCC human samples and involved cell proliferation, migration, invasion progress. SNHG17 promoted HCC cell growth and metastasis in vivo. Furthermore, we investigated the downstream factor of SNHG17, SNHG17 acted as a molecular sponge for miR-3180-3p, and SNHG17 regulated RFX1 expression via miR-3180-3p. SNHG17 promotes tumor-like behavior in HCC cells via miR-3180-3p/RFX1.

We determined RFX1 as the target of miR-3810-3p; SNHG17 enhanced the progression of HCC via the miR-3180-3p/RFX1 axis. Taken together, our findings may provide insight into the molecular mechanism involved in the progression of HCC and develop SNHG17 as a novel therapeutic target against HCC.

肝细胞癌 (HCC) 是最常见的癌症类型之一，与患者生活质量差和全球健康负担有关。参与 HCC 发展和进展的机制仍然知之甚少。

对肝细胞癌人类样品和细胞系进行 qRT-PCR 以进行表达评估。CCK-8法、Transwell迁移和侵袭法用于细胞功能检测。动物实验测定SNHG17对细胞生长的作用体内. 进行蛋白质印迹以评估细胞中EMT的水平。进行 RIP、RNA 下拉和荧光素酶报告基因分析以评估 SNHG17、miR-3180-3p 和 RFX1 之间的相关性。

我们的研究表明，SNHG17 在人类 HCC 样本中上调，并涉及细胞增殖、迁移、侵袭过程。SNHG17促进HCC细胞生长和转移体内. 此外，我们研究了 SNHG17 的下游因子，SNHG17 作为 miR-3180-3p 的分子海绵，SNHG17 通过 miR-3180-3p 调节 RFX1 的表达。SNHG17 通过 miR-3180-3p/RFX1 促进 HCC 细胞中的肿瘤样行为。

我们确定 RFX1 作为 miR-3810-3p 的靶标；SNHG17 通过 miR-3180-3p/RFX1 轴促进 HCC 的进展。总之，我们的研究结果可能有助于深入了解参与 HCC 进展的分子机制，并将 SNHG17 开发为针对 HCC 的新型治疗靶点。