The occurrence of adventitious roots is necessary for the survival of cuttings. In this study, comparative transcriptome analysis between two ramie (Boehmeria nivea L.) varieties with different adventitious root (AR) patterns was performed by mRNA-Seq before rooting (control, CK) and 10 days water-induced adventitious rooting (treatment, T) to reveal the regulatory mechanism of rooting. Characterization of the two ramie cultivars, Zhongzhu No 2 (Z2) and Huazhu No 4 (H4), indicated that Z2 had a high adventitious rooting rate but H4 had a low rooting rate. Twelve cDNA libraries of the two varieties were constructed, and a total of 26,723 genes were expressed. In the non-water culture condition, the number of the distinctive genes in H4 was 2.7 times of that in Z2, while in the water culture condition, the number of the distinctive genes in Z2 was nearly 2 times of that in H4. A total of 4411 and 5195 differentially expressed genes (DEGs) were identified in the comparison of H4CK vs. H4T and Z2CK vs. Z2T, respectively. After the water culture, more DEGs were upregulated in Z2, but more DEGs were downregulated in H4. Gene ontology (GO) functional analysis of the DEGs indicated that the polysaccharide metabolic process, carbohydrate metabolic process, cellular carbohydrate metabolic process, cell wall macromolecule metabolic process, and photosystem GO terms were distinctively significantly enriched in H4. Simultaneously, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that photosynthesis, photosynthesis antenna proteins, and starch and sucrose metabolism pathways were distinctively significantly enriched in H4. Moreover, KEGG analysis showed that jasmonic acid (JA) could interact with ethylene to regulate the occurrence and number of AR in Z2. This study reveals the transcriptomic divergence of two ramie varieties with high and low adventitious rooting rates, and provides insights into the molecular regulatory mechanism of AR formation in ramie.

中文翻译：

---

比较转录组分析为Ram麻不定根形成的分子调控机制提供了新的见解。

不定根的出现对于插条的生存是必要的。在这项研究中，两个two麻（Boehmeria nivea）之间的比较转录组分析在生根前（对照，CK）和10天水诱导的不定根生根（处理，T），通过mRNA-Seq进行了具有不同不定根（AR）模式的变种，以揭示生根的调控机制。中zhu 2号（Z2）和华株4号（H4）这两个麻品种的特征表明，Z2不定根的生根率高，而H4的生根率低。构建了两个变种的十二个cDNA文库，共表达了26,723个基因。在非水培养条件下，H4的独特基因数量是Z2的2.7倍，而在水培养条件下，Z2的独特基因数量是H4的近2倍。在H4CK与H4T和Z2CK与Z2T的比较中，共鉴定出4411和5195个差异表达基因（DEG），分别。水培后，Z2中更多的DEG被上调，而H4中更多的DEG被下调。DEG的基因本体（GO）功能分析表明，多糖代谢过程，碳水化合物代谢过程，细胞碳水化合物代谢过程，细胞壁大分子代谢过程和光系统GO术语在H4中显着丰富。同时，《京都基因与基因组百科全书》（KEGG）分析显示，光合作用，光合作用触角蛋白以及淀粉和蔗糖代谢途径明显富集于H4。此外，KEGG分析表明，茉莉酸（JA）可以与乙烯相互作用，调节Z2中AR的发生和数量。