Abstract

To search for antifungal leads, the metabolites of an insect-derived fungus Fusarium lateritium ZMT01 were investigated, providing five sesquiterpenes (1–5), including new molecules microsphaeropsisins D and E (1 and 2). The evaluated antifungal activities in vitro which are higher than the positive control triadimefon include: 1 and 2 towards Fusarium oxysporum (MICs 50, 25 mg L⁻¹; triadimefon 100 mg L⁻¹); 1, 2, 4 and 5 towards Penicillium italicum (MICs 25, 12.5, 25, 25; triadimefon 50 mg L⁻¹), 1, 2 and 4 towards Colletotrichum musae (MICs 25, 12.5, 25; triadimefon 80 mg L⁻¹), 2 and 4 towards Fusarium graminearum (MICs 100, 100; triadimefon 150 mg L⁻¹). The bioassay in vivo displayed that the banana anthracnose control effect of 2 (100 mg L⁻¹) was also higher than that of triadimefon (Inhibition ratios 27.5 ± 2.5%, 55.3 ± 1.4%, 52.1 ± 1.3% for 2, 22.5 ± 2.1%, 47.2 ± 2.0%, 36.6 ± 2.2% for triadimefon at 4 d, 8 d and 12 d, respectively).

摘要

为了寻找抗真菌先导，研究了昆虫衍生真菌Fusarium lateritium ZMT01 的代谢物，提供了五种倍半萜 ( 1-5 )，包括新分子微球菌素 D 和 E ( 1和2 )。评估的体外抗真菌活性高于阳性对照三唑酮包括：1和2对尖孢镰刀菌（MICs 50, 25 mg L ^-1；三唑酮 100 mg L ^-1）；1 , 2 , 4和5朝向意大利青霉（MIC 25、12.5、25、25；三唑酮 50 mg L ^-1）、1、2和4对炭疽菌（ MIC 25、12.5、25；三唑酮 80 mg L ^-1）、2和4对禾谷镰刀菌（MIC 100、100；三唑酮 150 mg L ^-1 )。体内生物测定表明， 2（100 mg L ^-1 ）的香蕉炭疽病防治效果也高于三唑酮（2的抑制比为27.5±2.5%、55.3±1.4%、52.1±1.3% ）, 22.5 ± 2.1%, 47.2 ± 2.0%, 36.6 ± 2.2%，三唑酮分别在 4 天、8 天和 12 天）。