Abstract

One new 3,5-dimethylorsellinic acid (DMOA)-based meroterpenoid (1), one prenylated tryptophan derivative (2), together with ten known compounds (3−12) were isolated from the endophytic fungus Aspergillus sp. from Tripterygium wilfordii. Their structures and absolute configurations were determined by NMR spectroscopic data, HRESIMS data, UV and IR data as well as electronic circular dichroism (ECD) calculation. In structure, compound 1 was a rare example of DMOA-based meroterpenoid with a cis-fused C/D ring system, and compound 2 possessed an unusual (E)-oxime group. In bioactivity, the lovastatin analogues 5, 6, 9 and 10 showed potential immunosuppressive activity against anti-CD3/anti-CD28 monoclonal antibodies (mAbs)-irritated murine splenocytes proliferation, with IC₅₀ values ranging from (5.30 ± 0.51) μM to (16.51 ± 1.62) μM.

摘要

从内生真菌Aspergillus sp.中分离出一种新的基于 3,5-二甲基羟色胺酸 (DMOA) 的类二萜 ( 1 )、一种异戊二烯化色氨酸衍生物 ( 2 ) 以及十种已知化合物 ( 3-12 ) 。来自雷公藤。它们的结构和绝对构型由核磁共振光谱数据、HRESIMS 数据、紫外和红外数据以及电子圆二色性 (ECD) 计算确定。在结构上，化合物1是具有顺式稠合 C/D 环系统的基于 DMOA 的二萜类化合物的罕见例子，化合物2具有不寻常的 ( E)-肟基团。在生物活性方面，洛伐他汀类似物5、6、9和10对抗CD3/抗 CD28 单克隆抗体 (mAbs) 刺激的小鼠脾细胞增殖具有潜在的免疫抑制活性，IC 50值_范围为 (5.30 ± 0.51) μM至(16.51 ± 1.62)微米。