The endoplasmic reticulum (ER) is one of the largest cytoplasmic organelles in eukaryotic cells and plays a role in many cellular processes, such as the production and quality control of secretory protein, lipid synthesis, and calcium homeostasis. The ER cannot be generated de novo, and thus its proper inheritance during cell division is paramount to the health and survival of the daughter cells. Although previous work has uncovered the cytoskeletal components involved, we still lack a comprehensive understanding of the intricate steps of and the cytoplasmic and membrane-bound components involved in ER inheritance. To directly address these issues, we utilized microfluidics and genetic analyses to show that before nuclear migration, early ER inheritance can be further divided into three distinctive steps. Moreover, we demonstrated that perturbing each of these steps affects the cell’s ability to mitigate ER stress. Thus, proper ER inheritance is essential to ensuring a healthy, functional cell.

内质网 (ER) 是真核细胞中最大的细胞质细胞器之一，在许多细胞过程中发挥作用，例如分泌蛋白的生产和质量控制、脂质合成和钙稳态。ER 不能从头生成，因此它在细胞分裂过程中的正确遗传对于子细胞的健康和存活至关重要。尽管以前的工作已经揭示了涉及的细胞骨架成分，但我们仍然缺乏对 ER 遗传中涉及的细胞质和膜结合成分的复杂步骤的全面了解。为了直接解决这些问题，我们利用微流体和遗传分析表明，在核迁移之前，早期 ER 遗传可以进一步分为三个不同的步骤。此外，我们证明扰动这些步骤中的每一个都会影响细胞减轻 ER 压力的能力。因此，适当的 ER 遗传对于确保健康、功能性细胞至关重要。