Our study (1) was designed to provide definitive direct data to the microglia community that PLX5622 does not act solely on microglia but also affects other peripheral macrophage populations. Many microglia papers have assumed or claimed that CSFR1 inhibition has no to minimal effects on peripheral immune cells.* The nonmicroglia effects of CSFR1 inhibition have direct ramifications for the interpretation of relevant experimental data; peripheral monocytes/macrophages engraft permanently into the retina after PLX5622 treatment (2, 3), causing remodeling and functional changes that affect studied outcome.

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回复 Green 和 Hume：PLX5622 短期 CSF1R 抑制的非小胶质细胞外周免疫效应 [生物科学]

我们的研究 ( 1 ) 旨在为小胶质细胞群提供明确的直接数据，即 PLX5622 不仅作用于小胶质细胞，而且影响其他外周巨噬细胞群。许多小胶质细胞论文已经假设或声称CSFR1抑制对外周免疫细胞没有或最小的影响。* CSFR1 抑制的非小胶质细胞效应对相关实验数据的解释有直接影响；在 PLX5622 治疗后，外周单核细胞/巨噬细胞永久植入视网膜 ( 2 , 3 )，导致重塑和功能变化，从而影响研究结果。