Structural comparison of two ferritins from the marine invertebrate Phascolosoma esculenta,FEBS Open Bio

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Structural comparison of two ferritins from the marine invertebrate Phascolosoma esculenta
FEBS Open Bio ( IF 2.8 ) Pub Date : 2021-01-15 , DOI: 10.1002/2211-5463.13080
Tinghong Ming _{1,

2} , Hengshang Huan _{1,

3} , Chang Su ₄ , Chunheng Huo _{1,

2} , Yan Wu _{1,

3} , Qinqin Jiang _{1,

3} , Xiaoting Qiu ₃ , Chenyang Lu _{1,

2} , Jun Zhou _{1,

2} , Ye Li _{1,

2} , Xiurong Su _{1,

2}

Affiliation

For marine invertebrates with no adaptive immune system, ferritin is a major intracellular iron‐storage protein with a critical role in innate immunity. Here, we present the crystal structures of two novel ferritins [Fer147 and Phascolosoma esculenta ferritin (PeFer)] from the marine invertebrate P. esculenta, which resides in muddy‐bottom coastal regions. Fer147 and PeFer exhibit the 4‐3‐2 symmetry of cage‐like hollow shells containing 24 subunits, similar to other known ferritins. Fer147 and PeFer contain both the conserved ferroxidase center and threefold channels. Subtle structural differences in the putative nucleation sites suggest possible routes of metal ion movement in the protein shells. However, the marked variation in the electrostatic potential of the threefold channels in Fer147 and the fourfold channels in PeFer suggests significant diversity between Fer147 and PeFer in terms of metal ion aggregation and cation exclusion. In summary, the presented crystal structures may serve as references for studies of the iron‐storage mechanism of additional ferritins from marine invertebrates.

中文翻译：

来自海洋无脊椎动物 Phascolosoma esculenta 的两种铁蛋白的结构比较

对于没有适应性免疫系统的海洋无脊椎动物，铁蛋白是一种主要的细胞内储铁蛋白，在先天免疫中起关键作用。在这里，我们展示了来自海洋无脊椎动物P. esculenta的两种新型铁蛋白 [Fer147 和Phascolosoma esculenta ferritin (PeFer)]的晶体结构, 居住在泥泞的沿海地区。Fer147 和 PeFer 表现出包含 24 个亚基的笼状空心壳的 4-3-2 对称性，类似于其他已知的铁蛋白。Fer147 和 PeFer 包含保守的亚铁氧化酶中心和三重通道。假定的成核位点的细微结构差异表明金属离子在蛋白质壳中运动的可能途径。然而，Fer147 中三重通道和 PeFer 中四重通道的静电势的显着变化表明 Fer147 和 PeFer 之间在金属离子聚集和阳离子排斥方面存在显着差异。总之，所呈现的晶体结构可作为研究来自海洋无脊椎动物的额外铁蛋白的铁储存机制的参考。

更新日期：2021-03-04

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