The human induced pluripotent stem cells (hiPSCs) derived cardiomyocytes (CMs) (hiPSC-CMs) retain the same genetic information as the donor, and they have been shown to faithfully recapitulate the disease phenotypes of various genetic cardiac diseases.

The hiPSC-CMs can be utilized in multiple types of studies and in most cases, the functionality of hiPSC-CMs is of interest. For the functional analyses, the hiPSC-CMs need to be manipulated after differentiation, e.g. enriched or dissociated into single-cell stage. For the functional assessments to be reliable and reproducible, the cell culture environment should support the cells in an optimal manner.

The aim of the present study was to evaluate the effect of various differentiation methods, as well as coating materials used for the dissociated cells on the functionality of the differentiated hiPSC-CMs.

The different protocols not only had different differentiation efficiencies, but they also yielded functionally different hiPSC-CMs. Additionally, the coating material had a major effect on the functionality of the hiPSC-CMs.

The results of the present study emphasize that the cardiac differentiation method and the coating material have a major effect on hiPS-CMs’ characteristics. Thus, when different hiPSC lines and results obtained in different labs are compared, extra care should be taken to check the conditions when results are compared.

人类诱导的多能干细胞（hiPSC）衍生的心肌细胞（CM）（hiPSC-CM）保留了与供体相同的遗传信息，并且已被证明能够忠实地概括各种遗传性心脏病的疾病表型。

hiPSC-CM可以用于多种类型的研究，并且在大多数情况下，hiPSC-CM的功能令人关注。对于功能分析，需要在分化后操纵hiPSC-CM，例如将其富集或解离成单细胞阶段。为了使功能评估可靠且可重现，细胞培养环境应以最佳方式支持细胞。

本研究的目的是评估各种分化方法的作用，以及用于离解细胞的涂层材料对分化的hiPSC-CMs功能的影响。

不同的协议不仅具有不同的区分效率，而且还产生了功能上不同的hiPSC-CM。此外，涂料对hiPSC-CM的功能有重大影响。

本研究的结果强调，心脏分化方法和涂层材料对hiPS-CMs的特性有重大影响。因此，当比较不同的hiPSC品系和在不同实验室中获得的结果时，在比较结果时应格外小心以检查条件。