A cell line was established from swim bladder of the Grass carp (Ctenopharyngodon idellus) (CiSB), which was permissive for infection and propagation of Grass Carp Reovirus (GCRV). CiSB cells displayed optimal growth at 27 °C using M199 medium containing 10% fetal bovine serum and a fibroblastic-like morphology. Karyotype analysis revealed that the average diploid chromosome number was 52 in 58% of cells at passage 60 compared to the wild type Grass carp cells (2n = 48). Infection with GCRV II isolate Hunan1307 was tracked by immunofluorescence and virus titration assay. The virus titer reached 10^5.2 TCID₅₀/mL on 7th days post infection (dpi). Healthy adult Grass carp that were challenged with the virus propagated onto CiSB cells, displayed the typical symptoms and histopathological changes of Grass carp hemorrhagic disease (GCHD). Therefore, the CiSB cells can be used to propagate GCRV II and serve as a useful tool to study the pathogenesis of GCHD.

从草鱼（Ctenopharyngodon idellus）（CiSB）的膀胱中建立了一个细胞系，该细胞系允许感染草鱼呼肠孤病毒（GCRV）和繁殖。使用含有10％胎牛血清和成纤维样形态的M199培养基，CiSB细胞在27°C下表现出最佳生长。核型分析显示，与野生型草鱼细胞（2n = 48）相比，在60代传代的58％细胞中平均二倍体染色体数为52。通过免疫荧光和病毒滴定法追踪GCRV II分离株Hunan1307的感染。病毒效价达到10 ^5.2 TCID ₅₀感染后第7天（dpi）/ mL。受病毒攻击的健康成年草鱼传播到CiSB细胞上，表现出草鱼出血性疾病（GCHD）的典型症状和组织病理学变化。因此，CiSB细胞可用于繁殖GCRV II，并可作为研究GCHD发病机理的有用工具。