Hypoxia is a critical issue in aquaculture which affects the metabolism and survival of fish. The critical oxygen tension (Pcrit), loss of equilibrium (LOE), respiration frequency (RF), plasma cortisol, glucose, lactate, cholesterol (CHO), triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), total protein, albumin, globulin, albumin/globulin, white blood cell (WBC), red blood cell (RBC), hemoglobin (Hb) and hematocrit (Hct) of turbot (male vs female) were determined during hypoxia and reoxygenation to evaluate their hypoxia tolerance and underlying physiological response. Results showed that dissolved oxygen (DO) values at Pcrit and LOE were 3.34 ± 0.17 mg/L and 1.30 ± 0.16 mg/L, respectively. RF significantly increased at Pcrit and LOE. Meanwhile, abnormal swimming behaviors were observed at DO values lower than 5 mg/L. Male and female showed no significant difference in above indexes. Hypoxia stress significantly increased plasma cortisol, glucose, and lactate contents at Pcrit and LOE, with the highest value obtained at LOE. Female plasma cortisol and glucose content were significantly higher than males at LOE, whereas the male lactate was significantly higher than females. Hypoxia stress did not affect the WBC number, plasma CHO, HDL, LDL levels and albumin/globulin of the male and female turbot. Plasma TG and total protein, albumin, globulin levels significantly increased at LOE and Pcrit, respectively. Female total protein significantly higher than males at Pcrit, but the opposite trend was found at LOE. RBC, Hb and Hct manifested similar cortisol results, the male and female showed no significant difference. The aforementioned parameters except for cortisol were recovered to normal levels after reoxygenation for 12 h. In conclusion, turbot manifest sex dimorphism difference during hypoxia stress-induced hematological and biochemical response.

低氧是水产养殖中的一个关键问题，它影响鱼类的代谢和生存。临界氧张力（Pcrit），平衡损失（LOE），呼吸频率（RF），血浆皮质醇，葡萄糖，乳酸，胆固醇（CHO），甘油三酸酯（TG），高密度脂蛋白（HDL），低密度脂蛋白（LDL），总蛋白，白蛋白，球蛋白，白蛋白/球蛋白，白细胞（WBC），红细胞（RBC），血红蛋白（Hb）和血细胞比容（Hct）在低氧和低氧期间测定复氧以评估其耐缺氧性和潜在的生理反应。结果表明，Pcrit和LOE的溶解氧（DO）值分别为3.34±0.17 mg / L和1.30±0.16 mg / L。Pcrit时射频显着增加和LOE。同时，在DO值低于5 mg / L时观察到异常游泳行为。男性和女性在上述指标上没有显着差异。低氧应激显着增加了Pcrit和LOE的血浆皮质醇，葡萄糖和乳酸含量，在LOE处获得最高值。在LOE时，女性血浆皮质醇和葡萄糖含量显着高于男性，而男性乳酸盐显着高于女性。低氧应激不影响雄性和雌性大菱t的白细胞数，血浆CHO，HDL，LDL水平和白蛋白/球蛋白。血浆TG和总蛋白，白蛋白，球蛋白水平在LOE和Pcrit分别显着增加。女性全蛋白在Pcrit上显着高于男性，但在LOE发现相反的趋势。RBC，Hb和Hct表现出相似的皮质醇结果，男性和女性无显着差异。再充氧12 h后，除皮质醇外，上述参数均恢复到正常水平。总之，在低氧应激引起的血液和生化反应过程中，大菱t表现出性别差异。