The prevalence of Pseudomonas species in freshwater fish and water from aquaculture farms in Turkey was determined on a monthly basis, in the period covering 2013–2017 and two seasons of 2018. The farms included in the study were located in six different regions of Turkey. A total of 90 Pseudomonas strains were isolated from water and diseased and healthy fish, and were classified under 20 species. The phenotypic characterization of the strains was based on oxidase and catalase activities, haemolysis, tolerance to temperature and sodium chloride, the production of fluorescent pigments and antibiotic resistance spectra. The phylogenetic identification of the 90 isolates was performed by a 4-gene multilocus sequence analysis, based on the partial sequencing of the 16S rRNA, gyrB, rpoB and rpoD genes. This is the first report on the isolation of several Pseudomonas species, namely, P. brenneri, P. defensor, P. haemolytica, P. lactis, P. lundensis, P. lurida, P. mandelii, P. meridiana, P. migulae, P. proteolytica, P. simiae, and P. weihenstephanensis, from freshwater salmonid fish. Two of these species, P. haemolytica and P. lactis have been isolated for the first time from fish farms, an environment quite different from their original isolation source, raw milk. Furthermore, seven putative new Pseudomonas species were isolated from water and farmed rainbow trout. During the 2013 to 2018 period, several Pseudomonas species were detected to have spread from the Aegean and Central Anatolia regions to the Eastern Anatolia and Black Sea regions.

每月一次确定土耳其淡水鱼和水产养殖场水中的假单胞菌种类的流行率，涵盖2013-2017年和2018年的两个季节。研究中所涉及的养殖场位于土耳其的六个不同地区。从水中以及患病和健康的鱼类中分离出总共90种假单胞菌菌株，归类为20种。菌株的表型表征基于氧化酶和过氧化氢酶活性，溶血作用，对温度和氯化钠的耐受性，荧光色素的产生和抗生素抗性谱。根据16S rRNA的部分测序，通过4基因多基因座序列分析对这90个分离物进行了系统发育鉴定，gyrB，rpoB和rpoD基因。这是关于几种假单胞菌属物种分离的第一份报告，即布氏假单胞菌，防御假单胞菌，溶血假单胞菌，乳酸假单胞菌，隆德毕生，假单胞菌，曼氏假单胞菌，子午线假单胞菌，形假单胞菌。 ，淡水鲑鱼鱼中的P. proteolytica，P。simiae和P. weihenstephanensis 。这些物种中的两个，溶血性疟原虫和乳酸性疟原虫，是首次从养鱼场中分离出来的，其环境与原始的分离来源生奶完全不同。此外，七个推定的新假单胞菌从水和养殖的虹鳟鱼中分离出该物种。在2013年至2018年期间，检测到数种假单胞菌属物种已从爱琴海和安纳托利亚中部地区扩散到安纳托利亚东部和黑海地区。