Secondary metabolites produced in glandular trichomes of tomato are involved in interactions with herbivores. In cultivated tomato (Solanum lycopersicum) glandular trichomes accumulate a blend of abundant monoterpenes and smaller amounts of a few sesquiterpenes. These mono- and sesquiterpenes are synthesized by three terpene synthases, TPS20 as well as TPS9 and TPS12, respectively. To study effects of these terpenes on performance and choice behavior of potato aphid (Macrosiphum euphorbiae), we utilized two tomato trichome mutants, hairless and odorless-2, that are differently affected in mono- and sesquiterpene production. Non-choice assays demonstrated that longevity and fecundity of M. euphorbiae were increased when kept on the trichome mutants. A principal component analysis of these aphid performance parameters and terpene production in the trichome mutants indicated that longevity and fecundity of M. euphorbiae were negatively correlated with production of the TPS12-derived sesquiterpenes β-caryophyllene and α-humulene. While we had previously shown that addition of pure β-caryophyllene/α-humulene to an artificial feeding diet affected M. euphorbiae apterae survivorship and feeding behavior, no such effects were observed here upon addition of a mixture of pure TPS20-derived monoterpenes. In olfactometer assays M. euphorbiae alates displayed differential choice behaviors towards the hairless and odorless-2 mutants suggesting a role of TPS20-derived monoterpenes in aphid attraction, which was further confirmed using a mixture of pure monoterpenes. Our analyses revealed contrasting roles of glandular trichome-derived terpenes in S. lycopersicum. While TPS12-derived sesquiterpenes contribute to host plant resistance against M. euphorbiae, TPS20-derived monoterpenes appear to be exploited as cue for host plant orientation by aphids.

番茄腺毛中产生的次生代谢产物与草食动物相互作用。在栽培番茄（茄属植物）中，腺毛积累了丰富的单萜和少量的倍半萜的混合物。这些单萜和倍半萜分别由三种萜烯合酶TPS20以及TPS9和TPS12合成。为了研究这些萜烯对马铃薯蚜虫（Macrosiphum euphorbiae）的性能和选择行为的影响，我们利用了两个番茄毛状体突变体，无毛和无味-2，它们在单萜和倍半萜的生产中受到不同的影响。非选择分析表明大戟念珠菌的寿命和繁殖力当保留在毛状体突变体上时，其增加。对蚜虫突变体中这些蚜虫性能参数和萜烯产量的主成分分析表明，大戟念珠菌的寿命和繁殖力与TPS12衍生的倍半萜β-石竹烯和α-α草烯的产生负相关。虽然我们先前已经证明，在人工喂养的饮食中添加纯β-石竹烯/α-腐草烯会影响大戟红掌杆菌的存活和喂养行为，但在添加纯TPS20衍生的单萜混合物后，未观察到这种影响。在嗅觉测定中，大戟念珠菌对无毛和无味2表现出不同的选择行为。突变体提示TPS20衍生的单萜在蚜虫引诱中的作用，使用纯单萜的混合物进一步证实了这一点。我们的分析揭示了在沙门氏菌中腺体毛状体萜烯的对比作用。TPS12衍生的倍半萜有助于宿主植物抵抗大戟念珠菌，TPS20衍生的单萜似乎被蚜虫用作宿主植物定向的线索。