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Diagnostics of Polymerus Hahn (Heteroptera, Miridae) Species Group Including Agricultural Pests in the East European Forest-Steppe, Based on Morphological and Molecular Characters
Entomological Review Pub Date : 2021-01-15 , DOI: 10.1134/s001387382006010x
V. B. Golub , M. Yu. Syromyatnikov , A. V. Kokina , V. A. Soboleva , E. V. Nesterova , V. N. Popov

Abstract

The paper presents an improved key to the co-occurring species of Polymerus Hahn, 1831 (Heteroptera, Miridae) based on morphological characters and our new data on the differences between the COI gene DNA sequences of these species. These characters, together with the data stored in the NCBI GenBank, provide the means for accurate and rapid identification of adults and nymphs of Polymerus unciniger Gapon, 2014, P. unifasciatus (Fabricius, 1794), P. cognatus (Fieber, 1858), P. vulneratus (Panzer, 1806), and P. brevicornis (Reuter, 1879). The subgenera Poeciloscytus Fieber, 1858 and Pachycentrum Gapon, 2014 differ considerably in morphological and molecular genetic characteristics. A PCR-RFLP method has been developed for rapid identification of the pest species P. vulneratus. The morphological characters of P. unifasciatus and the COI gene sequences of the forms earlier registered in GenBank as P. unifasciatus and P. microphthalmus (Wagner, 1951) reveal high intrapopulation variability. Polymerus microphthalmus may be a submacropterous form of P. unifasciatus with shortened hemelytra and smaller eyes, while the macropterous form of the latter species is characterized by larger eyes.



中文翻译:

基于形态和分子特征对包括东欧森林草原上的农业害虫在内的多哈种(Heteroptera,Miridae)物种组进行诊断

摘要

本文基于形态特征和我们关于这些物种的COI基因DNA序列之间差异的新数据,提供了对1831年Polymerus Hahn共存物种的改良钥匙(Heteroptera,Miridae)。这些字符以及存储在NCBI GenBank中的数据,为准确,快速地识别松材线虫加蓬Polymerus unciniger Gapon),2014。P . unifasciatus(Fabricius,1794),白兰地球菌P. cognatus)(Fieber,1858),P. vulneratus(Panzer,1806)和br。brevicornis(Reuter,1879)。Poeciloscytus Fieber亚属,1858年和Pachycentrum加蓬(Gapon),2014年在形态和分子遗传学特征上有很大差异。已经开发了PCR-RFLP方法以快速鉴定害虫物种P.vulneratus褐紫假单胞菌的形态学特征以及较早在GenBank中注册为单种假单胞菌微小眼球的形式的COI基因序列(Wagner,1951)显示出较高的种群内变异性。微小 眼球聚合物可能是短小亚目的P. unifasciatus体形,具有较短的血统和较小的眼睛,而后者的大型翼状体的特征是较大的眼睛。

更新日期:2021-01-15
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