Punicalagin is the most popular ellagitannin found in pomegranate husk and is well known to reduce the risk of cancer and cardiovascular diseases (CVDs). The present work describes a novel method for the preparation of nanoparticles of Punicalagin using pomegranate (Punica granatum) since the use of Punicalagin in bulk form is associated with many problems. In this study, nanoparticles of Punicalagin were synthesized, characterized using various techniques, and further evaluated for their antioxidant, antibacterial, and antiproliferative potential using HepG2 cancer cells. DLS analysis revealed that the average size of nanoparticles of Punicalagin was 87 nm, whereas SEM analysis confirmed spherical shaped nanoparticles with size ranging from 90 to 116 nm. HPLC studies confirmed the presence of Punicalagin in synthesized nanoparticles. The results also revealed that nanoparticles of Punicalagin were nearly four times more potent antioxidants than bulk and possessed an inhibition zone of about 13 mm. The response of antiproliferative assay showed that the nanoparticles of Punicalagin caused nearly 44% reduction while bulk form showed only a 15% reduction in cell viability of cancerous cells at 100 µg/mL. The study suggests a great potential for use of the herbal drug in nano form to treat cancer as compared to its bulk counterparts.

石榴皮素是在石榴皮中发现的最受欢迎的鞣花单宁，众所周知，它可以降低罹患癌症和心血管疾病（CVD）的风险。本工作描述了一种利用石榴（Punica granatum）制备石榴素纳米粒的新方法。），因为散装使用Punicalagin存在许多问题。在这项研究中，合成了Punicalagin的纳米颗粒，使用多种技术进行了表征，并使用HepG2癌细胞进一步评估了其抗氧化，抗菌和抗增殖的潜力。DLS分析表明，石榴碱的纳米颗粒的平均尺寸为87nm，而SEM分析证实球形纳米颗粒的尺寸为90-116nm。HPLC研究证实了在合成的纳米颗粒中存在石榴碱。结果还表明，石榴碱的纳米颗粒是强效抗氧化剂的几倍，比块状抗氧化剂强四倍，并具有约13 mm的抑制区。抗增殖试验的响应表明，在100 µg / mL浓度下，普尼卡拉金的纳米颗粒减少了近44％，而散装形式的癌细胞活力仅降低了15％。这项研究表明，与大量同类产品相比，纳米形式的草药具有巨大的治疗癌症的潜力。