The combined influence of cadmium (Cd) and drought was evaluated on in vitro regenerated plants of banana ‘Grand Nain’ at morpho-physiological and molecular levels. A significant reduction was observed due to separate or combined treatments of Cd and polyethylene glycol (PEG) in total chlorophyll, carotenoid content, and all growth parameters except root length, which was increased under Cd treatment. Meanwhile, other traits were increased significantly under combined treatment of Cd and PEG (1 and 3%), including malondialdehyde, phenolic content, and peroxidase specific activity. Furthermore, molecular analysis using the inter-simple sequence repeats (ISSR) markers confirmed the harmful effect of Cd. Cadmium caused DNA damage reflected by 18.4% polymorphism compared to the control. Out of 14 polymorphic bands, 12 were absent due to Cd treatment and two bands existed only in Cd-treated samples compared with control. However, the addition of PEG was efficient to reduce the harmful effect of Cd on DNA profile. In this regard, 1% PEG reduced the number of polymorphic bands produced by Cd to the half, while 3% PEG was enough to give an equal DNA profile to that of control with no polymorphic bands. In addition, there was no effect of the separate PEG treatments on ISSR pattern compared with control. Results confirmed the mutagenicity and toxicity of Cd on banana. The effect of PEG-induced drought reduced the damaging effects of Cd by minimizing its uptake and accumulation in plant tissues.

在体外评估了镉和干旱的综合影响在形态生理和分子水平上再生了香蕉“ Grand Nain”的植物。观察到由于单独或联合处理Cd和聚乙二醇（PEG）的总叶绿素，类胡萝卜素含量以及除根部长度以外的所有生长参数的显着降低，在Cd处理下其增加。同时，在Cd和PEG的组合处理下（1％和3％），其他特性显着提高，包括丙二醛，酚含量和过氧化物酶比活性。此外，使用简单序列间重复（ISSR）标记进行的分子分析证实了镉的有害作用。与对照组相比，镉引起的DNA损伤反映出18.4％的多态性。在14个多态性谱带中，由于Cd处理而没有12条，与对照相比，仅在Cd处理的样品中存在两个谱带。但是，添加PEG可以有效降低Cd对DNA谱的有害影响。在这方面，1％PEG将Cd产生的多态性条带数量减少一半，而3％PEG足以提供与没有多态性条带的对照相同的DNA谱。此外，与对照相比，单独的PEG处理对ISSR模式没有影响。结果证实了镉对香蕉的致突变性和毒性。PEG引起的干旱影响通过使Cd在植物组织中的吸收和积累最小化而降低了Cd的破坏作用。此外，与对照相比，单独的PEG处理对ISSR模式没有影响。结果证实了镉对香蕉的致突变性和毒性。PEG引起的干旱影响通过使Cd在植物组织中的吸收和积累最小化而降低了Cd的破坏作用。此外，与对照相比，单独的PEG处理对ISSR模式没有影响。结果证实了镉对香蕉的致突变性和毒性。PEG引起的干旱影响通过使Cd在植物组织中的吸收和积累最小化而降低了Cd的破坏作用。