Hymenocrater comprises of 11 species throught the world. The genus is an Irano-Turanian elements, which has several usages in traditional medicine. Northeast of Iran is the main geographical region of Hymenocrater species. The aims of the peresent study was to delimit 11 species of Hymenocrater to determine the species relationships based on morphological and molecular data (cp-DNA sequence), to produce divergence time and to construct barcode gap using the internal transcribed sequence because of adulteration of this genus in local markets. According to the results, Neighbor-Net network and UPGMA dendrogram of morphological data can delimit Hymenocrater species. Meanwhile, analyses of Chloroplast sequences (psbA-trnH) revealed that the species relationship and delimitation are difficult and complicated due to gene flow, hybridization, gene duplication, reticulation and chloroplast capture. Divergence time of Hymenocrater species estimated from Bayesian Evolutionary Sampling Trees date were seven million years ago. The center origin of Hymenocrater in Iran is Razavi Khorasan. Genetic fingerprinting of correctly identified medicinal species provides standard for comparing plant specimens sold in the market with true to type medicinal plant. Therefore, ITS sequences are important for barcoding this medicinally significant plant. It is correctly identified medicinal species, which will be used as standard for evaluating market sold plants.

处女膜足包括全世界11个物种。该属是伊朗-土耳其语元素，在传统医学中有多种用法。伊朗东北部是处女膜兽物种的主要地理区域。这项有意义的研究的目的是确定11个处女膜兽的种类，以便根据形态学和分子数据（cp-DNA序列）确定种类之间的关系，产生发散时间，并使用内部转录序列构建条形码间隙，因为这种掺假行为本地市场的属。根据结果​​，形态数据的Neighbor-Net网络和UPGMA树状图可以界定处女膜的种类。同时，分析叶绿体序列（psbA-trnH）表明由于基因流动，杂交，基因重复，网状化和叶绿体捕获，物种之间的关系和定界是困难和复杂的。根据贝叶斯进化取样树的日期估算的膜翅目动物的发散时间是七百万年前。处女膜主在伊朗的中心起源是拉扎维·霍拉桑。正确识别的药用物种的遗传指纹图谱提供了将市场上出售的植物标本与真正的药用植物进行比较的标准。因此，ITS序列对于对该药用重要植物进行条形码编码很重要。它是正确识别的药用物种，将用作评估市售植物的标准。