Abstract

The wavy hair-grass, Avenella flexuosa (L.) Drejer (also known as Deschampsia flexuosa (L.) Trin. or Lerchenfeldia flexuosa (L.) Schur), is a species of perennial herbaceous plant of the Poaceae family that is widespread in Eurasia, Africa, North and South America. Whether this species belongs to the Deschampsia genus has long been an open question. Taking into account that the 5S rDNA comparison is a convenient tool in the molecular taxonomy of plants, we cloned and sequenced this region of the A. flexuosa genome and evaluated the genetic distances between this species and other species of the Poeae tribe. It has been found that at least two structural classes of 5S rDNA repeats are present in the A. flexuosa genome. The spacer regions in the 5S rDNA of A. flexuosa and D. antarctica have a low level of similarity that does not exceed the level of similarity with representatives of several other genera of the Poeae tribe. The 5S rDNA clones of A. flexuosa and D. antarctica form two distinct clades on the phyllodendogram. Taking together, our results support the idea that A. flexuosa belongs to a separate genus Avenella.

中文翻译：

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5S核糖体DNA的分子组织和弯曲小菜蛾（禾本科）的分类学地位

摘要

波浪状的毛草，弯曲小花草（Avenella flexuosa（L.）Drejer（也称为Deschampsia flexuosa（L.）Trin。或Lerchenfeldia flexuosa（L.）Schur）是禾本科的多年生草本植物欧亚大陆，非洲，北美和南美。这个物种是否属于德尚普属一直是一个悬而未决的问题。考虑到该5S rDNA的比较是在植物的分子分类学的方便工具，我们克隆和测序在这个区域A. flexuosa基因组，并评估了此物种与其它物种的POEAE部落之间的遗传距离。已经发现在5S rDNA重复序列中至少存在两个结构类别。A. flexuosa基因组。弯曲弯曲杆菌和南极弯曲杆菌的5S rDNA中的间隔区具有很低的相似度，但不超过与Poeae部落其他几个属的相似度。弯曲弯曲杆菌和南极弯曲杆菌的5S rDNA克隆在叶状图上形成两个不同的进化枝。考虑在一起，我们的研究结果支持这一想法A. flexuosa属于一个独立的属Avenella。