Abstract

Carbon monoxide (CO) is considered to be an important molecule-gasotransmitter involved in the regulation of the functional activity of plants, including in the processes of adaptation to stress factors. However, the associations of CO with other participants of signaling in the plant cells remain poorly studied. Using an inhibitory method, the role of different calcium pools in realization of the influence of hemin (carbon monoxide donor) on generation and neutralization of reactive oxygen species (ROS) in the root cells of wheat (Triticum aestivum L.) plantlets and their resistance to damaging heating (45°C, 10 min) was studied. The treatment of plantlets with 5 μM hemin caused a transient increase in the activity of extracellular peroxidase in roots and an increase in the generation of ROS with the maximum in 1.5–2 h after the beginning of treatment. The chelator of extracellular calcium EGTA and the inhibitor of inositol-1,4,5-phosphate formation neomycin, which reduces calcium influx into the cytosol from intracellular compartments, almost completely eliminated an increase in the activity of extracellular peroxidase caused by exogenous CO. At the same time, EGTA (completely) and neomycin (partially) leveled an increase in the content of hydrogen peroxide in the roots of plantlets occurring under the influence of CO donor. The treatment of plantlets with hemin also induced an increase in the activity of superoxide dismutase, catalase, and intracellular peroxidase in roots. Both calcium antagonists eliminated these effects. In the presence of EGTA and neomycin, there was also no positive effect of the treatment of hemin on the state of biomembranes and survival of plantlets after the damaging heating. It was concluded that both extracellular calcium and that deposited in intracellular compartments is involved in CO donor-induced increase in the formation of ROS, induction of the antioxidant system, and development of heat resistance of wheat plantlets.

中文翻译：

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Hemin（一氧化碳供体）影响下小麦幼苗细胞氧化还原稳态和耐热性的钙依赖性变化

摘要

一氧化碳（CO）被认为是重要的分子-气体递质，参与调节植物的功能活动，包括在适应胁迫因素的过程中。然而，CO与植物细胞中信号传导的其他参与者的关联仍然研究不足。使用一种抑制方法，不同钙池在实现血红素（一氧化碳供体）对小麦（小麦）根细胞中活性氧物质（ROS）的生成和中和的影响中的作用研究了L.）幼苗及其对破坏性加热（45°C，10分钟）的抵抗力。用5μM血红素处理的小植株引起根部细胞外过氧化物酶活性的瞬时增加，并导致ROS的产生增加，在处理开始后的1.5–2 h达到最大值。胞外钙EGTA的螯合剂和肌醇-1,4,5-磷酸形成新霉素的抑制剂可减少钙从细胞内区室流入细胞质，几乎完全消除了外源性CO引起的细胞外过氧化物酶活性的增加。同时，EGTA（完全）和新霉素（部分）使受CO供体影响的幼苗根中过氧化氢含量增加。用血红素处理小植株还诱导根中超氧化物歧化酶，过氧化氢酶和细胞内过氧化物酶的活性增加。两种钙拮抗剂都消除了这些作用。在EGTA和新霉素的存在下，在破坏性加热后，处理血红素对生物膜的状态和幼苗的存活也没有积极作用。结论是，细胞外钙和细胞内隔室中的钙都参与了CO供体诱导的ROS形成增加，抗氧化剂系统的诱导以及小麦幼苗耐热性的发展。破坏性加热后，处理血红素对生物膜的状态和幼苗的存活也没有积极作用。结论是，细胞外钙和沉积在细胞内区室中的钙都与CO供体诱导的ROS形成增加，抗氧化剂系统的诱导以及小麦幼苗耐热性的发展有关。破坏性加热后，处理血红素对生物膜的状态和幼苗的存活也没有积极作用。结论是，细胞外钙和细胞内隔室中的钙都参与了CO供体诱导的ROS形成增加，抗氧化剂系统的诱导以及小麦幼苗耐热性的发展。