Valorization of cassava fibrous waste by lactic acid bacteria (LAB) into D-lactic acid (DLA) may assuage the massive supply chain for the synthesis of alternative bioplastics such as PLA. This investigation aims at developing a potential LAB cell factory say Lactobacillus delbrueckii, to synthesize DLA in a cost-effective biological route utilizing second-generation agricultural feedstock. Along with CFWEH (20 g/L), other complex nitrogen sources and yeast extract (YE)-based medium yielded 17.75 g/L of DLA. Further optimization based on one factor at a time approach (OFAT), YE at 5 g/L was found to be optimal for DLA production. At different initial CFWEH concentrations from 20 to 120 g/L, kinetic modeling of biomass and DLA formation and CFWEH consumption was carried out by weighted-average least square method. Predicted parameters show that the inhibitory concentration for substrate was above 99 g/L, also inhibition due to DLA synthesis occurred as high as 59 g/L for 120 g/L substrate loading. This research finding offers the knowledge of kinetic parameters, its transformation into operational parameters, which would be helpful for sustainable synthesis of DLA.

木薯纤维废料被乳酸菌（LAB）转化为D-乳酸（DLA）可能会缓解庞大的供应链，从而合成其他生物塑料（例如PLA）。这项研究的目的是建立一个潜在的LAB细胞工厂，例如德氏乳杆菌，以利用第二代农业原料的经济有效的生物途径合成DLA。除CFWEH（20 g / L）外，其他复杂的氮源和酵母提取物（YE）基培养基也可产生17.75 g / L的DLA。基于一个时间因素（OFAT）的进一步优化，发现以5 g / L的YE对DLA生产是最佳的。在从20到120 g / L的不同初始CFWEH浓度下，通过加权平均最小二乘法对生物量，DLA形成和CFWEH消耗进行动力学建模。预测的参数显示底物的抑制浓度高于99 g / L，并且由于载样量为120 g / L，DLA合成引起的抑制也高达59 g / L。这项研究发现提供了动力学参数，将其转化为运行参数的知识，