The aim of this study was to improve the quality of the micropropagated A. angustifolia Haw. plants cultured in temporary immersion bioreactors (TIS) comparing them with those produced through conventional semisolid-solid tissue culture system (SS). The Recipient for Automated Temporary Immersion (RITA^®) bioreactor was used as TIS in this work. The effect of different culture conditions, such as explants density, genotype, and duration of the incubation stages, were analyzed. The growth and morphological parameters measured for the in vitro cultured plants were: plant height, number of new leaves, number of shoots/explants, growth index (GI), dry mass content, and water content. In all experiments, it was observed that plantlets cultivated in the TIS grew larger than those cultivated in SS. Analyzing all the parameters used in this study, the results showed that RITA bioreactor generates a better shoot production and a better GI when using 20 plantlets per container. The number of shoots increased with time of culture (60 days) in both systems. However, the shoots and plantlets cultivated in TIS grew bigger and showed better quality (did not present necrosis in the leaves) than the ones cultured in SS. This study provides experimental evidence that the application of TIS for micropropagation of A. angustifolia is a viable option for the production of high-quality shoots for reforestation purposes.

本研究的目的是提高微繁殖的A. angustifolia Haw 的质量。在临时浸入式生物反应器 (TIS) 中培养的植物与通过传统半固体-固体组织培养系统 (SS) 生产的植物进行比较。自动临时沉浸式的接受者 (RITA ^®) 生物反应器在这项工作中用作 TIS。分析了不同培养条件的影响，例如外植体密度、基因型和孵化阶段的持续时间。测量的体外培养植物的生长和形态参数为：株高、新叶数、芽/外植体数、生长指数（GI）、干质量含量和含水量。在所有实验中，都观察到在 TIS 中培养的植株比在 SS 中培养的植株大。分析本研究中使用的所有参数，结果表明，当每个容器使用 20 个植物时，RITA 生物反应器产生更好的芽产量和更好的 GI。在两个系统中，枝条的数量随着培养时间（60 天）的增加而增加。然而，TIS 培养的嫩芽和幼苗比 SS 培养的长得更大，质量更好（叶片未出现坏死）。该研究提供了实验证据，证明 TIS 用于微繁A. angustifolia是生产用于重新造林的高质量枝条的可行选择。