As a common sequel to obesity, plasma and intracellular free fatty acid (FFA) concentrations are elevated and, as a consequence, manifold disturbances in metabolism may ensue. Biochemical processes in the cytosol and organelles, such as mitochondria and endoplasmic reticulum (ER), can be disturbed. In the ER, the maintenance of a high calcium gradient is indispensable for viability. In sarcoplasmic reticulum, selective FFA can induce endoplasmic reticulum stress by disrupting luminal calcium homoeostasis; however, there are limited studies in hepatic microsomes. Our studies found that FFA has a noxious effect on rat hepatic microsomal calcium flux, and the extent of which depended on the number of double bonds and charge. Furthermore, insofar as the FFA had no effect on microsomal calcium efflux, their inhibitory action primarily involves calcium influx. Finally, other cationic channels have been found in hepatic ER, and evidence is presented of their interaction with the Ca2+ ATPase pump.

中文翻译：

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肝内质网钙通量：游离脂肪酸和 KATP 通道参与的影响。

作为肥胖症的常见后遗症，血浆和细胞内游离脂肪酸 (FFA) 浓度升高，因此可能会导致代谢紊乱。细胞质和细胞器中的生化过程，如线粒体和内质网 (ER)，可能会受到干扰。在 ER 中，维持高钙梯度对于生存能力是必不可少的。在肌质网中，选择性 FFA 可通过破坏管腔钙稳态诱导内质网应激；然而，关于肝微粒体的研究有限。我们的研究发现 FFA 对大鼠肝微粒体钙通量有有害影响，其程度取决于双键和电荷的数量。此外，就 FFA 对微粒体钙外流没有影响而言，它们的抑制作用主要涉及钙内流。最后，在肝内质网中发现了其他阳离子通道，并提供了它们与 Ca2+ ATPase 泵相互作用的证据。