This paper reports a volatile organic compound (VOC) sensor based on olfactory receptors that were reconstituted into a lipid bilayer and used in a specifically designed gas flow system for rapid parts per billion (ppb)–level detection. This VOC sensor achieves both rapid detection and high detection probability because of its gas flow system and array design. Specifically, the gas flow system includes microchannels and hydrophobic microslits, which facilitate both the introduction of gas into the droplet and droplet mixing. We installed this system into a parallel lipid bilayer device and subsequently demonstrated parts per billion–level (0.5 ppb) detection of 1-octen-3-ol in human breath. Therefore, this system extends the various applications of biological odorant sensing, including breath diagnosis systems and environmental monitoring.

本文报道了一种基于嗅觉受体的挥发性有机化合物 (VOC) 传感器，该传感器被重组为脂质双层，并用于专门设计的气流系统中，用于快速检测十亿分之几 (ppb) 水平。这种VOC传感器由于其气流系统和阵列设计，实现了快速检测和高检测概率。具体来说，气流系统包括微通道和疏水微缝，它们有助于将气体引入液滴和液滴混合。我们将该系统安装到一个平行的脂质双层装置中，随后展示了人类呼吸中 1-octen-3-ol 的十亿分之一级 (0.5 ppb) 检测。因此，该系统扩展了生物气味传感的各种应用，包括呼吸诊断系统和环境监测。