Honey bee venom contains various enzymes with wide medical and pharmaceutical applications. The phospholipase A2 (PLA2) has been apparently purified from the venom of Egyptian honey bee (Apis mellifera lamarckii) 8.9-fold to a very high specific activity of 6033 U/mg protein using DEAE–cellulose and Sephacryl S-300 columns. The purified bee venom PLA2 is monomeric 16 kDa protein and has isoelectric point (pI) of 5.9. The optimal activity of bee venom PLA2 was attained at pH 8 and 45 °C. Cu2+, Ni2+, Fe2+, Ca2+, and Co2+ exhibited a complete activating effect on it, while Zn2+, Mn2+, NaN3, PMSF, N-Methylmaleimide, and EDTA have inhibitory effect. The purified bee venom PLA2 exhibited anti-platelet aggregation and anti-coagulation activities which makes it promising agent for developing novel anti-clot formation drugs in future.

中文翻译：

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埃及蜜蜂Apis mellifera lamarckii毒液中的磷脂酶A2酶具有抗血小板聚集和抗凝活性

蜜蜂毒液包含多种酶，具有广泛的医学和制药应用。使用DEAE-纤维素和Sephacryl S-300色谱柱，磷脂酶A2（PLA2）显然是从埃及蜜蜂（Apis mellifera lamarckii）的毒液中纯化得到的8.9倍至6033 U / mg蛋白的非常高的比活。纯化的蜂毒PLA2为单体16 kDa蛋白，等电点（pI）为5.9。蜂毒PLA2的最佳活性在pH 8和45°C下达到。Cu2 +，Ni2 +，Fe2 +，Ca2 +和Co2 +对其具有完全的活化作用，而Zn2 +，Mn2 +，NaN3，PMSF，N-甲基马来酰亚胺和EDTA具有抑制作用。纯化后的蜂毒PLA2具有抗血小板凝集和抗凝的作用，因此有望成为今后开发新型抗血凝形成药物的有前途的药物。