Apple replant disease (ARD) has been reported from all major fruit-growing regions of the world, and is often caused by biotic factors (pathogen fungi) and abiotic factors (phenolic compounds). In order to clarify the proteomic differences of Fusarium moniliforme under the action of phloridzin, and to explore the potential mechanism of F. moniliforme as the pathogen of ARD, the role of Fusarium spp. in ARD was further clarified. In this paper, the quantitative proteomics method iTRAQ analysis technology was used to analyze the proteomic differences of F. moniliforme before and after phloridzin treatment. The differentially expressed protein was validated by qRT-PCR analysis. A total of 4535 proteins were detected, and 293 proteins were found with more than 1.2 times (P< 0.05) differences. In-depth data analysis revealed that 59 proteins were found with more than 1.5 times (P< 0.05) differences, and most proteins were consistent with the result of qRT-PCR. Differentially expressed proteins were influenced a variety of cellular processes, particularly metabolic processes. Among these metabolic pathways, a total of 8 significantly enriched KEGG pathways were identified with at least 2 affiliated proteins with different abundance in conidia and mycelium. Functional pathway analysis indicated that up-regulated proteins were mainly distributed in amino sugar, nucleotide sugar metabolism, glycolysis/ gluconeogenesis and phagosome pathways. This study is the first to perform quantitative proteomic investigation by iTRAQ labeling and LC-MS/MS to identify differentially expressed proteins in F. moniliforme under phloridzin conditions. The results confirmed that F. moniliforme presented a unique protein profile that indicated the adaptive mechanisms of this species to phloridzin environments. The results deepened our understanding of the proteome in F. moniliforme in response to phloridzin inducers and provide a basis for further exploration for improving the efficiency of the fungi as biocontrol agents to control ARD.

中文翻译：

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基于ITRAQ的镰刀菌镰刀菌（Fusarium v​​erticillioides）对Phloridzin诱导剂的定量蛋白质组分析

世界上所有主要的水果种植地区都报告了苹果重植病（ARD），通常是由生物因子（病原真菌）和非生物因子（酚类化合物）引起的。为了阐明镰孢镰刀菌在镰刀菌作用下的蛋白质组学差异，并探讨镰刀菌作为ARD病原菌的潜在机制，以及镰刀菌的作用。在ARD中的进一步澄清。本文采用定量蛋白质组学方法iTRAQ分析技术分析了费洛津处理前后念珠状镰刀菌的蛋白质组差异。通过qRT-PCR分析验证了差异表达的蛋白质。总共检测到4535个蛋白质，发现293个蛋白质的差异超过1.2倍（P <0.05）。深入的数据分析表明，发现59种蛋白质的差异超过1.5倍（P <0.05），并且大多数蛋白质与qRT-PCR结果一致。差异表达的蛋白质影响了多种细胞过程，特别是代谢过程。在这些代谢途径中，共鉴定出8种显着富集的KEGG途径，其中至少有2种在分生孢子和菌丝体中具有不同丰度的相关蛋白。功能途径分析表明，上调的蛋白质主要分布在氨基糖，核苷酸糖代谢，糖酵解/糖异生和吞噬途径中。这项研究是第一个通过iTRAQ标记和LC-MS / MS进行蛋白质组学定量研究的方法，以鉴定在菲立津条件下念珠菌中差异表达的蛋白质。结果证实，念珠菌呈现出独特的蛋白质谱，表明该物种对phloridzin环境的适应机制。该结果加深了我们对响应磷霉素诱导物的念珠菌中蛋白质组的了解，并为进一步探索提高真菌作为控制ARD的生物防治剂的效率提供了基础。