Genetically accessible variation to some of the abiotic and biotic stresses are limited in the cultivated lentil (Lens culinaris Medik.) germplasm. Introgression of novel alleles from its wild relative species might be required for enhancing the genetic improvement of the crop. L. ervoides, one of the wild relatives of lentil, is a proven source of disease resistance for the crop. Here we introduce a lentil advanced backcross population (LABC-01) developed in cultivar CDC Redberry background, based on L. ervoides alleles derived from an interspecific recombinant inbred population, LR-59-81. Two-hundred and seventeen individuals of the LABC-01 population at BC2F3:4 generation were screened for the race 0 of anthracnose (Colletotrichum lentis) and stemphylium blight (Stemphylium botryosum) under controlled conditions. The population showed significant variations for both diseases and transfer of resistance alleles into the elite cultivar was evident. It also segregated for other traits such as days to flowering, seed coat colour, seed coat pattern and flower colour. Overall, we showed that LABC-01 population can be used in breeding programs worldwide to improve disease resistance and will be available as a valuable genetic resource for future genetic analysis of desired loci introgressed from L. ervoides.

中文翻译：

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扁豆×种之间的杂交产生了一个先进的小扁豆回交种群，用于未来的抗病性和基因组研究

在栽培的扁豆（Lens culinaris Medik。）种质中，某些非生物和生物胁迫的遗传可及变异是有限的。为了增强作物的遗传改良，可能需要从其野生近缘种中引入新的等位基因。扁豆的野生近缘种之一的紫苏乳酸菌是农作物抗病性的可靠来源。在这里，我们介绍了在CDC Redberry品种背景下开发的小扁豆先进回交种群（LABC-01），它基于来源于种间重组近交种群LR-59-81的L. ervoides等位基因。在受控条件下，筛选了BC2F3：4世代中LABC-01种群中的217位个体的炭疽病（Colletotrichum lentis）和枯萎病（Stemphylium botryosum）的第0种。种群显示出两种疾病的显着差异，并且抗性等位基因向优良品种的转移是明显的。它还针对其他特征进行了区分，例如开花天数，种皮颜色，种皮样式和花色。总体而言，我们证明了LABC-01种群可在世界范围内的育种计划中用于提高抗病性，并可作为宝贵的遗传资源，用于将来对从紫尾乳球菌渗入的所需基因座进行遗传分析。