Human bone marrow (BM) derived stromal cells contain a population of skeletal stem cells (SSCs), with the capacity to differentiate along the osteogenic, adipogenic and chondrogenic lineages enabling their application to clinical therapies. However, current methods, to isolate and enrich SSCs from human tissues remain, at best, challenging in the absence of a specific SSC marker. Unfortunately, none of the current proposed markers, alone, can isolate a homogenous cell population with the ability to form bone, cartilage, and adipose tissue in humans. Here, we have designed DNA-gold nanoparticles able to identify and sort SSCs displaying specific mRNA signatures. The current approach demonstrates the significant enrichment attained in the isolation of SSCs, with potential therein to enhance our understanding of bone cell biology and translational applications.

中文翻译：

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使用球形核酸富集人骨髓中的骨骼干细胞

人骨髓（BM）衍生的基质细胞包含一群骨骼干细胞（SSC），具有沿成骨，成脂和成软骨谱系分化的能力，使其能够应用于临床治疗。但是，目前从人组织中分离和富集SSC的方法充其量仍然是缺乏特异性SSC标记物的最大挑战。不幸的是，目前提出的任何标记都不能单独分离出具有形成人骨，软骨和脂肪组织能力的同质细胞群。在这里，我们设计了能够识别和分类显示特定mRNA签名的SSC的DNA-金纳米颗粒。当前的方法表明，分离SSC可以实现显着的富集，