Spot blotch disease caused by Bipolaris sorokiniana is a major constraint for wheat production in tropics and subtropics. The introgression of spot blotch resistance alleles to the disease susceptible lines is critical to securing the wheat production in these regions. Although genome-wide association studies (GWASs) for spot blotch were attempted earlier, the present study focused on identifying new quantitative trait loci (QTLs) for spot blotch under natural disease pressure in diverse field conditions. A total of 139 advanced spring wheat lines were evaluated in three environments (three years and two locations) in India and Bangladesh. The GWAS using 14,063 polymorphic genotyping-by-sequencing (GBS) markers identified eight QTLs associated with spot blotch disease resistance belonging to eight chromosomes across the wheat genome. Here, we report the identified marker–trait associations (MTAs), along with the allele effects associated with the disease. The functional annotation of the significant markers identified NBS-LRR, MADS-box transcription factor, and 34 other plant-related protein families across multiple chromosomal regions. The results indicate four promising new QTLs on chromosomes 1A (497.2 Mb), 1D (89.84 Mb), 2B (421.92 Mb), and 6D (6.84 Mb) associated with several disease resistance protein families. These results provide insights into new genomic regions associated with spot blotch disease, and with additional validation, could be utilized in disease resistance breeding efforts in wheat development.

斑斑病引起的 索氏双极是热带和亚热带小麦生产的主要限制因素。斑点抗性等位基因向易感病系的渗入对于确保这些地区的小麦产量至关重要。尽管较早尝试了斑点斑点的全基因组关联研究（GWAS），但本研究的重点是在自然病压力下，在不同田间条件下，为斑点斑点鉴定新的定量性状位点（QTL）。在印度和孟加拉国的三个环境（三年和两个地点）中，共评估了139条高级春小麦品系。GWAS使用14063个多态性序列基因分型（GBS）标记，鉴定了八个QTL，这些QTL与斑点斑点病抗性相关，属于整个小麦基因组的八个染色体。在这里，我们报告已识别的标记-性状关联（MTA），以及与疾病相关的等位基因效应。重要标记物的功能注释可识别NBS-LRR，MADS-box转录因子以及跨多个染色体区域的其他34个植物相关蛋白家族。结果表明，与几个抗病蛋白家族相关的1A（497.2 Mb），1D（89.84 Mb），2B（421.92 Mb）和6D（6.84 Mb）染色体上有四个有希望的新QTL。这些结果提供了与斑点斑点病相关的新基因组区域的见识，并具有额外的验证，可用于小麦发育中的抗病育种工作。和跨多个染色体区域的其他34个植物相关蛋白家族。结果表明，与几个抗病蛋白家族相关的1A（497.2 Mb），1D（89.84 Mb），2B（421.92 Mb）和6D（6.84 Mb）染色体上有四个有希望的新QTL。这些结果提供了与斑点斑点病相关的新基因组区域的见识，并具有额外的验证，可用于小麦发育中的抗病育种工作。和跨多个染色体区域的其他34个植物相关蛋白家族。结果表明，与几个抗病蛋白家族相关的1A（497.2 Mb），1D（89.84 Mb），2B（421.92 Mb）和6D（6.84 Mb）染色体上有四个有希望的新QTL。这些结果提供了与斑点斑点病相关的新基因组区域的见识，并具有额外的验证，可用于小麦发育中的抗病育种工作。