A change of an aspartic acid to asparagine of Taq (Thermus aquaticus) DNA polymerase is a gain of function mutation that supports faster PCR: the extension times for PCR amplification can be 2–3 times shorter. Surprising results from negative controls led to the discovery of strand-displacement ability and reverse transcriptase activity of Taq D732N DNA polymerase. We demonstrate that the mutant enzyme can, by itself, catalyze RT-PCR, and RT-LAMP assays. Residue 732 is on the surface of the enzyme, not near the active site.

中文翻译：

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Taq DNA 聚合酶的单一氨基酸变化可实现更快的 PCR、逆转录和链置换

将 Taq（水生栖热菌）DNA 聚合酶的天冬氨酸变为天冬酰胺是一种功能增益突变，支持更快的 PCR：PCR 扩增的延伸时间可以缩短 2-3 倍。来自阴性对照的令人惊讶的结果导致发现了 Taq D732N DNA 聚合酶的链置换能力和逆转录酶活性。我们证明突变酶本身可以催化 RT-PCR 和 RT-LAMP 检测。残基 732 位于酶的表面，不在活性位点附近。