Aedes aegypti, as one of the vectors transmitting several arboviruses, is the main target in mosquito control programs. Permethrin is used to control mosquitoes and Aedes aegypti get exposed due to its overuse and are now resistant. The increasing percentage of permethrin resistant Aedes aegypti has become an important issue around the world and the potential influence on vectorial capacity needs to be studied. Here we selected a permethrin resistant (p-s) Aedes aegypti population from a wild Florida population and confirmed the resistance ratio to its parental population. We used allele-specific PCR genotyping of the V1016I and F1534C sites in the sodium channel gene to map mutations responsible for the resistance. Two important factors, survival rate and vector competence, that impact vectorial capacity were checked. Results indicated the p-s population had 20 times more resistance to permethrin based on LD50 compared to the parental population. In the genotyping study, the p-s population had more homozygous mutations in both mutant sites of the sodium channel gene. The p-s adults survived longer and had a higher dissemination rate for dengue virus than the parental population. These results suggest that highly permethrin resistant Aedes aegypti populations might affect the vectorial capacity, moreover, resistance increased the survival time and vector competence, which should be of concern in areas where permethrin is applied.

中文翻译：

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埃及伊蚊对氯菊酯的耐药性影响媒介能力

埃及伊蚊作为传播多种虫媒病毒的媒介之一，是蚊虫控制计划的主要目标。氯菊酯用于控制蚊子，埃及伊蚊由于过度使用而暴露，现已产生抗药性。耐氯菊酯埃及伊蚊的比例不断增加已成为世界各地的一个重要问题，需要研究其对媒介能力的潜在影响。在这里，我们从佛罗里达州野生种群中选择了具有氯菊酯抗性（ps）的埃及伊蚊种群，并确认了与其亲本种群的抗性比率。我们使用钠通道基因中 V1016I 和 F1534C 位点的等位基因特异性 PCR 基因分型来绘制导致耐药性的突变图谱。检查了影响载体容量的两个重要因素：存活率和载体能力。结果表明，根据 LD50 计算，ps 群体对氯菊酯的耐药性是亲本群体的 20 倍。在基因分型研究中，ps群体在钠通道基因的两个突变位点上都有更多的纯合突变。ps 成虫比亲代种群存活时间更长，登革热病毒传播率更高。这些结果表明，高度抗氯菊酯的埃及伊蚊种群可能会影响媒介能力，此外，耐药性增加了存活时间和媒介能力，这在应用氯菊酯的地区应该引起关注。