Oxidative stress, caused by the excessive production of reactive oxygen species (ROS), results in cellular damage. Therefore, functional materials with antioxidant properties are necessary to maintain redox balance. Turmeric leaves (Curcuma longa L. leaves; TL) are known to have antioxidant properties, including 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2′-Azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), and Hydrogen peroxide (H₂O₂) radical scavenging activity in several studies. The antioxidant effects of TL come from distinct bioactive compounds, such as curcumin, total phenolic compounds, and flavonoids. Therefore, in this study, the antioxidant effects of a water extract of TL (TLE) against H₂O₂ treatment were assessed in vitro Vero cells and in vivo zebrafish models. The intracellular ROS generation and the proportion of sub-G1 phase cells were evaluated in H₂O₂- or/and TLE-treated Vero cells to measure the antioxidant activity of TLE. TLE showed outstanding intracellular ROS scavenging activity and significantly decreased the proportion of cells in the sub-G1 phase in a dose-dependent manner. Furthermore, cell death, ROS generation, and lipid peroxidation in the H₂O₂-treated zebrafish model were attenuated as a consequence of TLE treatment. Collectively, the results from this study suggested that TLE may be an alternative material to relieve ROS generation through its antioxidant properties or a suitable material for the application in a functional food industry.

中文翻译：

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姜黄叶提取物对过氧化氢诱导的Vero细胞体外和斑马鱼体内抗氧化作用的抗氧化作用。

由过量产生的活性氧（ROS）引起的氧化应激会导致细胞损伤。因此，具有抗氧化性能的功能材料对于维持氧化还原平衡是必要的。姜黄叶（姜黄叶，TL）已知具有抗氧化特性，包括2,2-二苯基-1-吡啶并肼基（DPPH），2,2'-叠氮基二（3-乙基苯并噻唑啉）-6-磺酸酸（ABTS）和过氧化氢（H ₂ O ₂）自由基清除活性的多项研究。TL的抗氧化作用来自不同的生物活性化合物，例如姜黄素，总酚类化合物和类黄酮。因此，在这项研究中，TL（TLE）水提取物对H ₂ O ₂的抗氧化作用评估了体外Vero细胞和体内斑马鱼模型的治疗效果。在H ₂ O ₂或/和TLE处理的Vero细胞中评估细胞内ROS的产生和亚G1期细胞的比例，以测量TLE的抗氧化活性。TLE显示出出色的细胞内ROS清除活性，并以剂量​​依赖性方式显着降低了sub-G1期细胞的比例。此外，H ₂ O ₂中的细胞死亡，ROS生成和脂质过氧化TLE处理的结果是减毒了斑马鱼模型。总体而言，这项研究的结果表明，TLE可能是通过其抗氧化特性减轻ROS生成的替代材料，或者是适用于功能食品行业的合适材料。