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Study of the Enzyme Activity Change due to Inkjet Printing for Biosensor Fabrication
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2021-01-13 , DOI: 10.1021/acsbiomaterials.0c01515 Yang Bai 1 , Dongxing Zhang 2, 3 , Qiuquan Guo 2 , Junfeng Xiao 2 , Mingyue Zheng 2 , Jun Yang 1, 2
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2021-01-13 , DOI: 10.1021/acsbiomaterials.0c01515 Yang Bai 1 , Dongxing Zhang 2, 3 , Qiuquan Guo 2 , Junfeng Xiao 2 , Mingyue Zheng 2 , Jun Yang 1, 2
Affiliation
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Enzymes, the most commonly used biosensing element, have a great influence on the performance of biosensors. Recently, drop-on-demand (DOD) printing technique has been widely employed for the fabrication of biosensors due to its merits of noncontact, less waste, and rapid deposition. However, enzyme printing studies were rarely conducted on the effect of printing parameters from the aspect of the pressure wave propagation mechanism. This study investigated the effects of pressure wave propagation on enzyme activity from the aspects of wave superposition, wave amplitude, resulting mechanical stress, and protein conformation change using pyruvate oxidase as the model enzyme. We found that the mechanical stress increased the activity of pyruvate oxidase during the inkjet printing process. A shear rate of 3 × 105 s–1 enhanced the activity by 14.10%. The enhancement mechanism was investigated, and the mechanical activation or mild proteolysis was found to change the conformation of pyruvate oxidase and improve its activity. This study is fundamental to understand the effect of both printing mechanism and induced mechanical stress on the properties of biomolecules and plays an important role in modulating the activity of other enzyme-based inks, which is crucial for the development of biosensors.
中文翻译：
喷墨印刷在生物传感器制造中酶活性变化的研究
酶是最常用的生物传感元件，对生物传感器的性能影响很大。近年来，按需滴（DOD）打印技术由于其非接触，浪费少和沉积迅速的优点而被广泛用于制造生物传感器。然而，从压力波传播机制的角度来看，很少进行酶印刷研究印刷参数的影响。本研究以丙酮酸氧化酶为模型酶，从波的叠加，波幅，产生的机械应力和蛋白质构象变化等方面研究了压力波传播对酶活性的影响。我们发现机械应力增加了喷墨打印过程中丙酮酸氧化酶的活性。剪切速率为3×10 5s –1将活性提高了14.10％。研究了增强机理，并发现机械活化或轻度蛋白水解作用可改变丙酮酸氧化酶的构象并提高其活性。这项研究对于了解印刷机理和机械应力对生物分子特性的影响至关重要，并且在调节其他基于酶的油墨的活性方面起着重要作用，这对于生物传感器的发展至关重要。
更新日期：2021-02-08
中文翻译：
喷墨印刷在生物传感器制造中酶活性变化的研究
酶是最常用的生物传感元件，对生物传感器的性能影响很大。近年来，按需滴（DOD）打印技术由于其非接触，浪费少和沉积迅速的优点而被广泛用于制造生物传感器。然而，从压力波传播机制的角度来看，很少进行酶印刷研究印刷参数的影响。本研究以丙酮酸氧化酶为模型酶，从波的叠加，波幅，产生的机械应力和蛋白质构象变化等方面研究了压力波传播对酶活性的影响。我们发现机械应力增加了喷墨打印过程中丙酮酸氧化酶的活性。剪切速率为3×10 5s –1将活性提高了14.10％。研究了增强机理，并发现机械活化或轻度蛋白水解作用可改变丙酮酸氧化酶的构象并提高其活性。这项研究对于了解印刷机理和机械应力对生物分子特性的影响至关重要，并且在调节其他基于酶的油墨的活性方面起着重要作用，这对于生物传感器的发展至关重要。
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